Abstract
Cryptosporidium spp. oocyst recovery in water and milk samples was evaluated. Samples were inoculated with a suspension of 1.2×10(7) Cryptosporidium spp. oocysts and submitted to centrifugal flotation, using different solutions (sucrose, NaCl, MgSO4, ZnSO4, AlSO4, NH4SO4 40% and NH4SO4 80%). Centrifugation of the samples was carried out in two stages for concentration using two methods that differed in the order in which the saturated solutions were used, namely only in the first stage of method I and only in the second stage of method II. Oocyst identification was performed using the Kinyoun and Koster histochemical staining techniques. Samples analyzed by method I showed different degree of oocyst recovery, namely 10.9% with NaCl and 42.5% with MgSO4 in water and milk samples, while those samples analyzed by method II showed 10.6% with NaCl and 5.3% with sucrose in water and milk, respectively. Histochemical staining methods have no influence on the degree of oocysts recovery. The efficiency of Cryptosporidium spp. oocysts recovery methods depends on the nature and composition of the sample and on the methodology used for oocyst concentration.
Highlights
Criptosporidiosis is an important disease related to public health
Samples (100ml) were inoculated with nonpurified Cryptosporidium spp. oocysts (LeChevallier et al, 1995) that were extracted from fecal suspension and kept in 10% formaldehyde
After inoculating 1.2×107 Cryptosporidium spp. oocysts, the samples were homogenized and distributed into glass tubes for centrifugation, which was performed at 206×g, for 10 minutes (Deng and Cliver, 1999) using two stages for both sample concentration and reconcentration by means of two different methods: method I suggested by Webster et al (1996) and method II proposed by Kageruka et al (1984) (Fig. 1)
Summary
The zoonotic potential of Cryptosporidium parvum is not very clear. It occurs in several species of animals and it increases the risk of infection by animal contact or by ingestion of contaminated food and water. The individual shows gastroenteric clinical symptoms that can be severe and even lead to death. It may happen if they are immunodeficient individuals, such as HIV positive patients. Self-limiting patients may act as asymtomatic carriers of great epidemiological interest (Lima et al, 2001). Epidemiological features of this parasitic protozoa lead to the assumption that the incidence of Cryptosporidium spp. in aquatic environment is underestimated. The lack of accurate proper methods for the detection of oocysts in water contributed to this report (Lima and Stamford, 2003)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
