Effectiveness of combined use of antibiotics, essential metals and probiotic bacterial strain complexes against multidrug resistant pathogens

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Large-scale uncontrolled use of antibiotics in various spheres of human activities (agriculture, veterinary medicine, and food industry) has led to the emergence of multi-resistant pathogen isolates. The aim of this study was to assess the complex potential effects of antibiotics and metals on P. aeruginosa clinical isolate. In this study, clinical isolates of P. aeruginosa were used as test organisms in respect of which combinations based on antibiotics, salts of essential metals with a high level of dissociation in aqueous solutions and the probiotic bacterial strain of B. subtilis 534 were examined. The specific selection criterion for antibiotics and metals was their resistance to specific concentrations of B. subtilis 534 strain and their inhibitory or subinhibitory effect against P. aeruginosa. In the course of the research it was found that the most promising is the use of CuSO4, since it has a more pronounced bactericidal effect on P. aeruginosa at a concentration of 40 mg/ml, in contrast to B. subtilis 534 for which this concentration is not toxic. Of all the studied antibacterial drugs, we selected fosfomycin for further research as it meets the requirements of the studies performed, with more pronounced resistance of B. subtilis 534 (0.25 mg/ml) compared to P. aeruginosa (0.03125 mg/ml). Generalized experimental data indicate that studies of the antibacterial complex based on fosfomycin, CuSO4, and the probiotic strain B. subtilis 534 are the most promising.