Effectiveness of Cinnamomum cassia against Liver and Kidney Biochemical Assay and Hematology in Bisphenol-A Induced Rats

Objective To evaluate the anti- herpes simplex virus type 1 (HSV-1) activity of KPC-rg1, a water extract from Cinnamomum cassia, and explore its potential function of broad-spectrum antivirus effect. Methods In vitro, the changes of morphology of Vero cells were assessed and viral loads were detected after cells were infected with HSV-1 alone and HSV-1 pre-treated with KPC-rg1 respectively. The corneal lesions of mouse and tree shrew corneal infection model were evaluated after they were infected with HSV-1 alone and HSV-1 pre-treated with KPC-rg1 respectively. The antiviral activity of KPC-rg1 against 9 viruses were measured by CPE and GFP reduction assays. Results The virus replication of HSV-1 infected cells was moderately inhibited by KPC-rg1 in a dose range of 0.0001-1.0 mg/ml, while the cells were completely protected when they were infected with HSV-1 pre-treated with KPC-rg1 (0.001-1.0 mg/ml). The corneal lesions of animals were improved in both mouse and tree shrews models infected with HSV-1 after the treatment of KPC-rg1, while animals were completely protected from infection when HSV-1 pre-treated with KPC-rg1. KPC-rg1 had a potential anti-virus effect on the enveloped viruses such as HSV-1, HCMV, RSV alone and HIV-1. Conclusions KPC-rg1 is a collosol (Tyndall effect) which would immediately form a stable super-nanoparticle structure of KPC-rg1/virus when encounter virus, and thus the virus coated by KPC-rg1 lost its ability of infection. KPC-rg1 can reduce the suffering by newly or latent virus infection because its encapsulation of virus and inhibition of further infection. Our study added additional proofs of the anti-viral property of the water extract from Cinnamomum cassia, and provided a further basis to develop KPC-rg1 as a drug which could be potentially applied in clinic to treat HSV-1 infection. Key words: Cinnamon water extract KPC-rg1; Herpes simplex virus type 1; Antiviral activity

Background: Serious seed-borne and soil-borne pathogens are existing at the rhizosphere area, many species of Pythium can cause seed rot and damping-off disease. The application of plant extracts and biological agents as alternative ways for the management of plant diseases has recently become an important variable component for Integrated Pest Management, so plant metabolites regarded as eco-friendly where plants play an important role in the control of diseases. The current study amid to evaluate the allelopathy effect of two species of biological agents particularly Trichoderma spp. and some plant extracts against the soil-borne pathogen P. aphanidermatum on cucumber seedlings under laboratory conditions. Methods: In this study laboratory investigation during 2018, used the allelopathy effect of two species of Trichoderma (T. harzianum and T. hamatum) and five plant extracts, in Three conc. 10.000, 20.000 and 30.000 ppm, to control damping-off disease in cucumber caused by Pythium aphanidermatum instead of using chemicals fungicides. Result: Our study observed that Trichoderma spp., have a high inhibitory effect on the tested pathogen, but in the assay of plant extracts, only some with different concentrations had a significant effect against P. aphanidermatum (in-vitro). Cinnamomum cassia, Syzygium aromaticum and Eucalyptus globulus were most affective from all other five tested plant extracts, where the concentration of 30.000 ppm gave the highest inhibitory effect against P. aphanidermatum, while Psidium guajava and Lawsonia inermis extracts showed a lower inhibitory effect on radial growth of P. aphanidermatum.

Syringaresinol from Cinnamomum cassia (L.) J.Presl ameliorates cognitive deficits and AD pathology via AMPK in a DM-AD mouse model.

The essential oil from the twigs of Cinnamomum cassia Presl alleviates pain and inflammation in mice