Effectiveness of Brucellosis on Polymorphisms of IL-1B and IL-10 Concentrations Among Iraqi Patients

Abstract

In this study, (60) blood samples were taken from patients infected with brucellosis, and (60) samples were taken from healthy individual as a control group. The patients were visiting Emam Ali Hospital for Fever in Baghdad city, during the period from 1st February to 1st November 2023. The results of gender distribution showed that among patients infected with Brucellosis, 30 (50.0%) were males and 30 (50%) were females, while among the control group, 31(51.7%) were males and 29(48.3%) were females. Also the distribution of infections according residency were matched between urban and rural locations 30(50.0%) for both. While the distribution of Brucellosis among age groups showed that the highest incidence of infection was in the age groups (40-50) years and (17-29) years 22(36.7%) for each, followed by the age group (30-39) years 16(26.7%). The mean levels of anti Brucella antibodies IgM, IgG, IL-10, IL-1B in patients with brucellosis were (0.11±0.17), (0.09±0.15), (5.62±1.99), (4.92±1.76) respectively compared to their mean levels in the control group (2.01±1.17), (1.33±0.59), (60.68±26.81), (22.85±9.17) respectively with highly significant differences (p<0.01). The ROC test showed that the Sensitivity of Brucella IgM concentration was100% and Specificity 100% in Cut off >0.84. Also the Sensitivity of Brucella IgG concentration was 100% and Specificity 100% in Cut off >0.33. Also the Sensitivity of IL-10 concentration was 100% and Specificity 100% in Cut off >18.04, and the Sensitivity of IL-1B concentration was 100% and Specificity 100% in Cut off >9.08. There were mutations occurred with IL-1B gene ID 3553 in SNPs, rs1143627. The variation of wild TT was changed to CC, CC, TC, TC, CC, AG, CC, TC in 8 cases out of 11 in comparison with the control group, and the mutation occurred with IL-1B gene ID 3553 in SNPs, rs1143627. The variation of wild TT was changed to CC, CC, TC, TC, CC, AG, CC, TC in 8 cases out of 11 in comparison with the control group.