Effectiveness of Bacillus clausii and Bacillus megaterium on some Physiological and Biochemical Parameters in Rats That Induced Diarrhea

Abstract

The current work aimed to isolate and diagnose the species of the genus Bacillus, investigate their susceptibility as prebiotics, and study their therapeutic effect in vivo in induced infection with Staphylococcus aureus in terms of their effect on blood picture parameters. These parameters include red blood cell count, hemoglobin concentration, and lipid profile. The lipid profile includes the total cholesterol and lipoproteins, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) triglycerides, and protein parameters (concentrations of total protein, globulin and albumin) in male rats which were compared with control groups after 28 days. This work includes 20 male Albino Sprague-Dawley rats, aged 8-9 weeks, and average weights of 148 g. They were randomly divided into five groups and four replicates per group. Two types of probiotics of the genus Bacillus were identified, Bacillus claussi and Bacillus megaterum. The results showed that the infection induced by Staphylococcus aureus caused a significant rise (P<0.05) in the white blood cells and platelets (17.37 and 650.0) IU/Liters compared to the control group, and a significant decrease in the number of red blood cells at 5.040 mm3 and hemoglobin concentration (11.300 g/dt). The findings showed that cholesterol, triglycerides and LDL concentrations rose, which were (68.07, 89.00 and 17.90) and a decrease in high-density lipoproteins (HDL) (33.25) compared with the control group. The protein standardssignificantly decreased at probability (P<0.05). In the concentration of total protein and albumin, it recorded (3.600 and 4.00) dL/g compared to the control group (5.400 and 4.80) dL/g. There were no significant differences in the globulin protein between the different groups. Yet, adding Bacillus species to the infected group of rats had a significant effect in the reduction of the negative influence on all parameters measured in the experiment.