Effectiveness of anti-pressure protective mask for medical personnel fighting against coronavirus disease 2019

Objective To find a safer and more effective facial care method, reduce the incidence rate of facial skin injury, and improve patients' comfort level, as well as work efficiency of nursing. Methods 90 patients with orotracheal intubation were divided into the control group, transparent dressing group and skin protecting membrane group by random number table method. The control group was conducted with basic facial nursing; the transparent dressing group was applied with protective transparent dressing on the basis of basic facial nursing; the skin protecting membrane group was applied with skin protecting membrane on the basis of basic facial nursing. This research was conducted by observing and recording the incidence rate of skin injury, severity of skin injury, patients' comfort level, duration of skin care and the results. Results The incidence rate of facial skin injury was as follows: skin protecting membrane group 6.89% (2/29)and transparent dressing group 15.15% (5/33)were better than the control group 50.00% (14/28), statistical significance was found among the three groups (χ2=16.75,P < 0.05); The times of facial skin nursing was as follows: the skin protecting membrane group (4.28±0.53) times was less than transparent dressing group (4.52±0.76) times and the control group (6.04±1.35) times, statistical significance was found among the three groups (Hc=32.65, P < 0.05). Patients' comfort level comparison were as follows: the skin protecting membrane group was better than transparent dressing group and the control group, statistical significance was found among the three groups (Hc=22.42,P < 0.05);Duration of skin care were as follows: skin protecting membrane group (10.40±1.35) minutes was less than transparent dressing group (14.09±1.27) minutes and the control group (12.35 ±1.28) minutes, statistical significance was found among the three groups (F=85.87, P< 0.05). Conclusions Skin protective membrane can effectively reduce the incidence and severity of facial skin injury on patients with orotracheal incubation, improve patients' comfort level, and greatly reduce operation time for nurses through its easer operation and less time consumption. Key words: Facial skin injury; Orotracheal incubation; Skin protective membrane; Clinical research

To determine the environmental contamination degree of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in corona virus disease 2019 (COVID-19) wards, to offer gui-dance for the infection control and to improve safety practices for medical staff, by sampling and detecting SARS-CoV-2 nucleic acid from the air of hospital wards, the high-frequency contact surfaces in the contaminated area and the surfaces of medical staff's protective equipment in a COVID-19 designated hospital in Wuhan, China. From March 11 to March 19, 2020, we collected air samples from the clean area, the buffer room and the contaminated area respectively in the COVID-19 wards using a portable bioaerosol concentrator WA-15. And sterile premoistened swabs were used to sample the high-frequency contacted surfaces in the contaminated area and the surfaces of medical staff's protective equipment including outermost gloves, tracheotomy operator's positive pressure respiratory protective hood and isolation clothing. The SARS-CoV-2 nucleic acid of the samples were detected by real-time fluorescence quantitative PCR. During the isolation medical observation period, those medical staff who worked in the COVID-19 wards were detected for SARS-CoV-2 nucleic acid with oropharyngeal swabs, IgM and IgG antibody in the sera, and chest CT scans to confirm the infection status of COVID-19. No SARS-CoV-2 nucleic acid was detected in the tested samples, including the 90 air samples from the COVID-19 wards including clean area, buffer room and contaminated area, the 38 high-frequency contact surfaces samples of the contaminated area and 16 surface samples of medical staff's protective equipment including outermost gloves and isolation clothing. Moreover, detection of SARS-CoV-2 nucleic acid by oropharyngeal swabs and IgM, IgG antibodies in the sera of all the health-care workers who participated in the treatment for COVID-19 were all negative. Besides, no chest CT scan images of medical staff exhibited COVID-19 lung presentations. Good ventilation conditions, strict disinfection of environmental facilities in hospital wards, guidance for correct habits in patients, and strict hand hygiene during medical staff are important to reduce the formation of viral aerosols, cut down the aerosol load, and avoid cross-infection in isolation wards. In the face of infectious diseases that were not fully mastered but ma-naged as class A, it is safe for medical personnel to be equipped at a high level.

Objective To observe the effect of two endotracheal-tube methods and to seek better endotracheal-tube method. Methods Totally 60 orotracheal intubation patients from September to December 2015 in neurology intensive care unit (NICU) were randomly divided into the experimental group and the control group. Patients in the experimental group were given new type endotracheal intubation fixed bracket, while patients in the control group were given tape fixed method. We compared the condition of facial skin allergy and injury, effect of tracheal intubation fixed method, and the incidence of ventilator associated pneumonia of patients in two groups. Results In the experimental group, the incidence rate of facial skin irritation and damage was 6.7%; the incidence rate of displacement of endotracheal tube was 6.7%, and they were both lower than the data in the control group (χ2=35.749, 28.081; P 0.05). Conclusions The new type of endotracheal intubation fixed method can significantly reduce the incidence rate of facial allergy and damage. It is safe and effective, and it also can increase the patient′s oral comfort. It is worthy of clinical promotion. Key words: Tracheal intubation; Fixed method; Facial injury

Objective To study the protective function of nimodipine on facial nerve injury and its effect on the expression of glial cell line-derived neurotrophic factor (GDNF). Methods Ninety-six SD rats were randomly divided into sham-operated group, facial nerve injury group, nimodipine pretreatment group, and nimodipine post-treatment group. Rat models of facial nerve injury in thc later 3groups were established. The dynamic changes of expression of GDNF were observed by HE staining and Western blotting in different treatment groups and at different time points (1, 3 and 6 months after the injury). Restdts Compared with the facial nerve injury group, the nimodipine pretreatment and post-treatment groups had significantly less severe nerve damage and significantly up-rcgulated expression of GDNF (P＜0.05). The expression of GDNF in the nimodipine pretreatment group was statistically higher than that in the nimodipine post-treatment group (P＜0.05). However, the expression of GDNF in the nimodipine post-treatment group was not statistically different from that in the facial nerve injury group 3 and 6 months after the injury (P＞0.05). Conclusion Nimodipine has significant facial nerve protective effect, and one of the mechanisms of nimodipine to protect the facial nerve is to regulate the GDNF expression. Key words: Facial nerve; Nimodipine; Glial cell line-derived neurotrophic factor

Objective To explore the application effect of nosting barrier film in the skin nursing of laryngeal carcinoma patients with trachea cannula after surgery. Methods Totally 90 cases of laryngeal cancer patients after radiotherapy with tracheal cannula, were randomly divided into three groups, with 28 cases in the control group, 33 cases in the dressing group, and 29 cases in the skin protective film group. Patients in the control group were treated with basic nursing method including using saline to clean plastic casing, cleaning and disinfecting skin, using sterile gauze to cover the field of radiation skin and plaster fixation; dressing group adopted Comfeel hydrocolloid dressings on the basis of basic nursing method; skin protective film group adopted Genetime spray skin protective film to protect skin of radiation field on the basis of basic nursing. After radiotherapy, the incidence of skin injury, severity of skin injury, patient comfort, and the time and times of changing skin dressing everyday were observed and compared among three groups. Results The incidence of radiation skin injury in the skin protective film group (6.89%) and dressing group (15.15%) , were all lower than the control group (50%) (χ2=16.75, P<0.05) ; the times of changing skin dressing everyday in the skin protective film group (4.28±0.53) times/d and the dressing group (4.52±0.76) times/d, were lower than the control group (6.04±1.35) times/d (Hc=32.65, P<0.05) ; patient comfort in the skin protective film group and the dressing group was superior to the control group (Hc=22.42, P<0.05) ; the time of changing skin dressing in the skin protective film group (10.40±1.35) min, was less than the dressing group (14.09±1.27) and the control group (12.35±1.28) min (F=85.87, P<0.05) . Conclusions The skin protective film can effectively reduce the incidence of skin injury in patients with laryngeal cancer and radiotherapy, improve patient comfort, reduce the labor time of nurses, and improve the quality of care and efficiency. Key words: Laryngeal neoplasms; Skin care; Radiotherapy; Tracheal cannula

Objectives To analyze the clinical characteristics of fracture in elderly patients during the outbreak of Corona Virus Disease 2019 (COVID-19). Methods Retrospective collection of data was performed on elderly patients (65 years and older) with fractures in the Third Hospital of Hebei Medical University between January 20 and February 19 2020, who were defined as case group. While, patients admitted between 2019 January 31 and March 2 (corresponding to the lunar calendar and 2020) were allocated as control group, with comparison to the case group in term of gender, age, fracture type, merge disease, injury factors, treatment, preoperative waiting time, hospitalization days and the perioperative complications. Results A total of 134 elderly patients with fractures were collected in this study, including 53 in the case group, with 19males and 34 females (M∶F=1∶1.79) and an average age of 81±8 years, and 81 cases in the control group,with 23 males and 58 females ( M∶F=1∶2.52) and an average age of 79±8 years. The proportion of patients with history of cerebral infarction in the case group (30 cases, 56.60%) was higher than that in the control group (29 cases, 35.80%, χ2= 5.625, P<0.05), and the proportion of patients falling down (53 cases, 100%) is higher than that of the control group (71 cases, 87.65%, χ2= 5.396, P<0.05). The proportion of patients with fractures caused by standing instability in the case group (18 cases, 33.96%) was higher than that in the control group (8 cases, 11.27%, χ2= 9.432, P<0.05), and the proportion of patients with fractures caused by bathroom slipping (15 cases, 28.30%) was higher than that of the control group (6 cases, 8.45%, χ2= 8.501, P<0.05). During perioperative period, the preoperative waiting time in the observation group [5 (4,7.25) d] was longer than that in the control group [4 (3,5) d, P<0.05], and the length of hospital stay [12 (9,15) d] was longer than that of the control group [10 (6,13) d, P<0.05]; The incidence of pulmonary infection in the case group (36 cases, 67.92%) was higher than that in the control group (16 cases, 19.75%, χ2= 31.306, P<0.05),and the incidence of deep vein thrombosis (31 cases, 58.49%) was higher than that of the control group (24 cases, 29.63%, χ2= 11.028, P<0.05). Conclusions This study suggests that the number of new fracture patients admitted to our hospital during the epidemic period is significantly lower than the same period last year. The elderly with cerebrovascular disease is the primary population for prevention of fracture, and home was the most predominant place. Due to the prolonged preoperative waiting time and hospital stay, the inpatients should focus on the prevention of perioperative pulmonary infection, deep venous thrombosis and other complications. Key words: Aged; Fractures; Epidemiology; COVID-19

Objective To design and practice standardized treatment protocols of large vestibular schwannomas (VS) based on the strategy of multidisciplinary treatment (MDT), and to discuss its clinical outcomes. Methods We analyzed all cases of unilateral large VS pathologically confirmed who underwent MDT at Department of Neurosurgery, Huashan Hospital, Fudan University from January 2010 to March 2016. Tumors with the maximal diameter longer than 3 cm were defined as large. The clinical data and follow-up results were retrospectively reviewed. A total of 266 VS patients were enrolled into this study. Electrophysiological monitoring, psychology assessment and facial nerve rehabilitation training were performed for all patients. One-stage anastomosis was applied for broken facial nerve. Results Gross total resection of the tumor was achieved in 261 patients (98.1%), near-total resection in 5 patients (1.9%), and the mortality rate was 0%. Facial nerve was anatomically preserved in 248 cases (93.2%). The function of facial nerve was evaluated according to House-Brackmann (H-B) scale, which showed that 125 patients (47.0%) were in grade Ⅰ-Ⅱ, 70 cases (26.3%) in grade Ⅲ, 71 cases (26.7%) in grade Ⅳ-Ⅵ within 1 month (short-term outcome). Perioperative complications included disequilibrium (10.1%), intracranial infection (8.6%), tinnitus (4.1%), lower cranial nerve injury (2.6%) and cerebrospinal fluid leak (1.1%). Follow-up data were available for 252 out of 266 patients (94.7%). For short-term facial outcome of 252 patients, there were 119 cases (47.2%) in grade Ⅰ-Ⅱ, 68 cases (27.0%) in grade Ⅲ, 65 cases (25.8%) in grade Ⅳ-Ⅵ. Meanwhile, long-term facial outcome showed that 184 patients (73.0%) were in grade Ⅰ-Ⅱ, 36 cases (14.3%) in grade Ⅲ, and 32 cases (12.7%) in grade Ⅳ-Ⅵ (P<0.01). There were 193 cases with serviceable hearing before surgery, of whom 60 cases showed serviceable hearing after surgery. The hearing preservation rate was 31.1%. Conclusions The MDT strategy for large VS could help improve the gross total resection rate and protect cranial nerve function. In addition, it might reduce perioperative mortality and the incidence of complications. Key words: Neuroma, acoustic; Microsurgery; Facial nerve injuries; Multidisciplinary treatment

Objective To explore the effects of hydrocolloid dressing on preventing nasotracheal intubation related nasal alar pressure injury among patients with orthognathic surgery. Methods From November 2016 to September 2017, we selected 450 patients with orthognathic surgery in Peking University School and Hospital of Stomatology by the study design of synchronous randomized controlled trial. All of the patients were divided into observation group and control group with the random number table, 225 cases in each group. Control group carried out nasotracheal intubation fixed by conventional method. Observation group received fixing by hydrocolloid dressing. We compared the incidence of nasal alar pressure injury of patients between two groups. Results The incidence of nasal alar pressure injury of patients in observation group and control group was 4.44%(10/225) and 14.22%(32/225) respectively. The incidence of observation group was lower than that of control group with a statistical difference (χ2=12.710, P<0.01). Conclusions Nasotracheal intubation fixed by hydrocolloid dressing helps to protect the skin of nasal alar among patients with orthognathic surgery to reduce the incidence of nasal alar pressure injury. Key words: Intubation, intratracheal; Pressure ulcer; Bandages, hydrocolloid; Nasal alar; Nasotracheal intubation

Objective To understand the clinical characteristics of patients with Corona Virus Disease 2019 (COVID-19) so as to further provide practical instructions for clinical practice through collecting and analyzing the clinical data of COVID-19 patients admitted to Beijing YuoAnHospital. Methods The clinical data were summarized and analyzed for COVID-19 classified as severe patients and critically ill patients between January 28, 2020 and February 12, 2020 in Beijing You An Hospital,including their age, gender, course of disease, epidemiological history, previous history, clinical symptoms, laboratory tests, CT findings of lungs, treatment and clinical outcomes. Statistical processing was performed using SPSS software (version 22.0). Results 19 patients were included in this study and divided into two groups by investigators, i.e. The percentage of patients was 10 for the severe type and 9 for the critically ill type. The average age of the patients was 75 years (range from 58 to 94 years), including 10 males and 9 females. Their course of diseases varied from 1 days to 14 days with a median of 5 days. Among these patients, 4 patients did used to reside in Wuhan, 13 patients had been in close contact with confirmed patients (including family gatherings, meals, living in the same room, etc.), and 2 patients had no relevant epidemiological history. 14 patients suffered from underlying chronic diseases (including 11 cases of hypertension, 4 cases of coronary heart disease, 4 cases of cardiac insufficiency, 3 cases of chronic obstructive pulmonary disease, 2 cases of previous anterior myocardial infarction, 3 cases of arrhythmia, 1 case of heart valve disorders, 2 cases of diabetes mellitus, 2 cases of chronic renal disease, etc.), and 4 patients were accompanied with 3 or more underlying diseases at the same time. The first symptoms at onset of illness were fever in 18 cases, cough in 13 cases, and dyspnea in 8 cases. 17 patients were accompanied with absolute lymphocyte count (ALC) lower than the normal range. 11 patients experience different degrees of hepatic injury, 10 patients had renal injury, 7 patients had abnormal myocardial enzymes in a varying degree. C-reactive protein was increased in 18 patients.12 patients received hormone therapy, 4 patients were treated with gamma globulin, 3 patients used non-invasive ventilator mechanical ventilation, and 7 patients used an invasive ventilator to assist ventilation. Moreover, 1 patients received continuous renal replacement therapy (CRRT), and 1 patient was treated with extracorporeal membrane oxygenation (ECMO). As of February 17, 2020, 10 patients had recovered or been discharged upon treatment, 4 patients were still in the intensive care unit (ICU), and 5 patients had died. Conclusions The elderly and individuals accompanied with underlying diseases were considered as independent predictors for developing severe and critically ill population, and were faced with increased mortality. The decrease of ALC and the increase of C-reactive protein levels in the patients, and the abnormality of liver function, renal function and myocardial enzyme spectrum in different degrees suggests the correlation with severe cases. Hormone and ventilator are effective in the treatment of severe cases. Key words: 2019-nCoV; clinical features; COVID-19; Critically ill diseases

To investigate the facial nerve contralateral innervation of patients with facial nerve injury. Electroneuronography (ENoG) of 22 university student volunteers (US) without any injury and systemic diseases and 108 patients with facial nerve injury were measured. The patients were divided into three groups: iatrogenic, traumatic, and Bell's palsy groups. When stimulating the facial nerve at the site below the lobule and behind the ramus, ENoGs of each branch of the facial nerve on ipsilateral and contralateral sides were recorded. A total of 76 branch I, 81 branch II, 88 branch III, and 66 branch IV were measured. (1) There were no significant differences among three patient groups (P > 0.05). (2) The ratios of contralateral innervation of the branch I and II of the patients were significantly. greater than those of the US group (P < 0.001). (3) The ratio of contralateral innervation of the branch I was greater than that of the branch II in the patients (P < 0.001). The contralateral innervation of the facial nerve increases after facial nerve injured.

Objective To investigate the clinical application value of preoperative facial nerve reconstruction by diffusion tensor imaging (DTI) in operations of acoustic neuroma. Methods We retrospectively collected 38 cases of patients who underwent operations of acoustic neuroma at Department of Neurosurgery, General Hospital of Tianjin Medical University from September 2013 to September 2015. Prior to operations, each patient underwent 3 T magnetic resonance DTI scanning. The facial nerve fiber tractography was made and the tumor was reconstructed with the software of 3D-Slice. Intraoperative electrophysiological monitoring was conducted to assess the degree of resection and facial nerve injury. Results Preoperative DTI reconstruction of the facial nerve was satisfactory in 32 (84.2%) out of 38 cases and clearly revealed the three-dimensional relationship between the facial nerve and the tumor, which was consistent with intraoperative verification. The facial nerve in 6 cases(15.8%)failed to be reconstructed by DTI tracer technique. DTI showed that the facial nerve was located at the ventral anterior inferior part in 10 (31.3%) cases, ventral central part in 16 (50.0%) cases, ventral anterior superior part in 5 (15.6%) cases and lower part of the tumor in 1 (3.1%) case. The facial nerve was anatomical preserved during operation in 36 (94.7%) cases. At 7 days post surgery, the facial nerve function was graded according to the House-Brackmann scale as 10 cases in grade Ⅰ, 20 cases in grade Ⅱ, 3 cases in grade Ⅲ and 5 cases in grade Ⅳ-Ⅴ; and cases with good facial nerve function accounted for 78.9% of all patients. Conclusion Preoperative reconstruction of the facial nerve by DTI could be used to guide resection of the acoustic neuroma and reduce the injury of facial nerve to a certain extent. Key words: Neuroma, acoustic; Facial nerve; Diffusion tensor imaging; Fiber tracking

COVID-19 in Kidney Transplantation: Outcomes, Immunosuppression Management, and Operational Challenges.

Objective To investigate the clinical characteristics, diagnosis and treatment of neurological diseases combined with coronavirus disease 2019(COVID-19). Methods The data of five patients of neurological diseases combined with COVID-19 (including two suspected cases) in the Department of Neurology, Renmin Hospital of Wuhan University from January 1, 2020 to February 24, 2020 were retrospectively collected. The epidemiology, clinical manifestations, laboratory and radiologic characteristics, diagnosis, treatment and outcomes of these cases were described and analyzed. Results Among the five patients, three were confirmed cases and two were suspected cases with a history of indirect contact with confirmed cases of COVID-19. The age of these patients was (76.4 ± 9.3) years. Neurological diseases included cerebral infarction in two cases, cerebral hemorrhage, Guillain-Barre syndrome and Alzheimer's disease each in one case. There was one acute case and one chronic case, and three patients were in the sequelae stage of acute diseases. One patient had an open airway as a result of Guillain-Barre syndrome. There were four cases of limb paralysis, half of whom were hemiplegic and quadriplegic respectively. The initial symptom was fever in all cases, and they showed a decrease in lymphocyte count. Four patients with chest computed tomographic scans showed bilateral lung lesions, of whom two cases showed multiple lung patches and ground glass shadows. Tests for 2019 novel coronavirus nucleic acid were positive in three patients and negative in two. The time from onset of COVID-19 to diagnosis was (6.6 ± 5.6) d. As of February 24, two patients died and the remaining were still hospitalized. Conclusions Neurological diseases, especially in elderly patients with limb paralysis, open airways and stroke, can be complicated by COVID-19, of which the prognosis is poor. Neurologists need to identify this situation early and pay attention to strengthening protection. Key words: Coronavirus disease 2019; Neurological diseases; Clinical characteristics

Objective To compare the therapeutic effect and complications of surgical treatment and conservative treatment for mandibular condylar fractures. Methods 100 cases of mandibular condylar fractures were selected as the research subjects.According to the different treatment method, the patients were divided into the observation group and the control group, 50 cases in each group.The observation group was treated by operation, and the control group was treated with conservative treatment.The therapeutic effect, mouth opening of before and after treatment, the change of movement and lateral movement, and complications were observed and compared between the two groups. Results The excellent and good rate of the observation group was 88%, compared with the control group of 86%, the difference was not statistically significant (P>0.05).12 months after treatment, mouth opening, protrusion movement and lateral movement of two groups were significantly improved than treatment after 6 months[(38.23±9.65) vs (35.98±9.65), (37.98±9.65) vs (35.43±8.76), (7.98±1.66) vs (7.21±1.03), (7.87±1.43) vs (6.98±1.06), (8.96±1.32) vs (8.06±1.06), (8.86±1.25) vs (8.86±1.25)], the differences were statistically significant (t=2.87, 3.43, 3.43, 3.43, 4.17, 4.21, all P 0.05). The incidence rate of postoperative complications including limitation of mouth opening, opening deflexion, joint symptoms, injury of facial nerve, parotid salivary fistula and bite axis disorder between two groups had no significant difference (16.0% vs 14.0%, P>0.05). Conclusion Surgical treatment and conservative treatment are the common methods for treatment of mandibular condylar fractures, and the effect of surgical treatment is better than conservative treatment. Key words: Mandible; Condyle; Fracture; Surgical treatment; Conservative treatment

To explore the effects of cervical sympathetic block (CSB) on facial nerve regeneration in rats and seek the optimal timing of treatment. A total of 72 adult Sprague-Dawley rats were divided randomly into 4 groups of sham-operation (sham), model control (NS), CSB1 and CSB2 (n = 18 each). Except for sham group, the model of peripheral facial paralysis was established for three other groups. After treating facial nerves injury with CSB at different timepoints, the behavioral changes of rats were observed.Electroneurography (ENoG) of injured nerves was performed and the expressions of glial cell derived neurotrophic factor (GDNF) of facial nerve nucleus were detected at different stages. The facial whisker movement function scores in sham, NS, CSB1 and CSB2 groups were 3.87 ± 0.35, 0.50 ± 0.52, 1.07 ± 0.62 and 0.81 ± 0.42 (F = 2.934, P = 0.035) at Day 7, 3.85 ± 0.37, 0.91 ± 0.52, 1.57 ± 0.65 and 1.07 ± 0.62 (F = 2.537, P = 0.038) at Day 14 and 3.85 ± 0.37, 1.71 ± 0.47, 3.00 ± 0.68 and 2.36 ± 0.49 (F = 3.627, P = 0.024) at Day 28. At Day 7, LatSD and AmpSD were not detected in NS, CSB1 and CSB2 groups.However, at Day 14, the values of LatSD were (1.26 ± 0.19), (6.67 ± 0.36), (4.67 ± 0.36) and (6.17 ± 0.36) ms, showing a significant difference (F = 3.052, P = 0.024) and AmpSD (6.42 ± 1.93), (2.16 ± 1.87), (4.16 ± 1.80) and (3.66 ± 1.40) mv, also a significance (F = 3.634, P = 0.021). And at Day 28, LatSD were (1.31 ± 0.17), (3.17 ± 0.19), (1.93 ± 0.12) and (2.60 ± 0.22) ms (F = 2.729, P = 0.032) and AmpSD (6.82 ± 2.30), (4.72 ± 5.23), (6.22 ± 3.50) and (5.82 ± 4.10) mv (F = 3.827, P = 0.019). The expression quantities of GDNF in 4 groups produced significant differences at Day 7 (4.67 ± 0.81, 13.52 ± 0.58, 26.17 ± 1.01 and 14.86 ± 1.03, F = 3.637, P = 0.028), Day 14 (5.67 ± 0.57, 24.41 ± 2.86, 26.52 ± 1.36 and 25.48 ± 1.42, F = 2.946, P = 0.031) and Day 28 (5.37 ± 0.92, 26.64 ± 1.68, 27.38 ± 1.66 and 25.69 ± 1.99, F = 4.273, P = 0.012). During early stage of facial nerve injury, the treatment of CSB may increase the expression of GDNF of facial nerve nucleus at early stage and thus accelerate the recovery of facial nerve injury in rats.