Effectiveness of anesthesia solution (2-Phenoxyethanol) in the transportation of brood stock of Perkasa Striped Catfish (Pangasianodon hypophthalmus Sauvage, 1878)

Abstract

This study aimed to obtain the optimal concentration of 2-phenoxy ethanol in the transportation medium for the brood stock candidate of the Perkasa Striped catfish. To determine the optimal concentration of 2-phenoxy ethanol in the transportation medium for the Perkasa Striped catfish brood stock candidate, the study used a completely randomized design with three replications consisting of five levels of treatment with a concentration of 2-phenoxy ethanol, namely 0 (control), 100, 150, 200, and 250 ppm. The test fish were Perkasa Striped catfish brood stock candidates weighing an average of 1,000 grams. A plastic bag of 80 x 40 cm was filled with 15 liters of water, 45 liters of oxygen, and 5 fish, and the transport test was carried out in a closed and static way for 12 hours. Parameters observed included survival rate at the end of transportation and on day 7 after transportation, the quality of the media water (dissolved oxygen, pH, temperature), and cortisol level in the blood. The results showed that 100 ppm of 2-phenoxy ethanol was the best concentration for transporting Perkasa Striped catfish brood stock candidates, with a survival rate of 100.0±0.0 percent at the end of transportation and 83.3±28.9 percent on the seventh day after transportation, dissolved oxygen content of 1.47±0.99 mg/L, and blood cortisol levels of 47.85±11.85 g/dL.