Effectiveness of a recombinant human follicle stimulating hormone on the ovarian follicles, peripheral progesterone, estradiol-17β, and pregnancy rate of dairy cows.

Mohamed Ali,Moustafa M. Zeitoun

doi:10.14202/vetworld.2016.699-704

Mohamed Ali, Moustafa M. Zeitoun

Open Access

https://doi.org/10.14202/vetworld.2016.699-704

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Journal: Veterinary world	Publication Date: Jul 1, 2016
Citations: 4	License type: cc-by

Affiliation: Qassim University

Abstract

Aims:This study aimed at elucidating the effects of recombinant human follicle stimulating hormone (r-hFSH) on the ovarian follicular dynamics, progesterone, estradiol-17β profiles, and pregnancy of dairy cows.Materials and Methods:Three groups (G, n=5 cows) of multiparous dairy cows were used. G1 (C) control cows were given controlled internal drug release (CIDR) and prostaglandin F2α; G2 (L) cows were given low dose (525 IU and G3 (H) cows were given high dose (1800 IU) of r-hFSH on twice daily basis at the last 3 days before CIDR removal. All cows were ultrasonically scanned for follicular growth and dynamics, and blood samples were collected every other day for two consecutive estrus cycles for the determination of estradiol-17β and progesterone.Results:Estrus was observed in all C and L but not in H cows. Dominant follicle was bigger in L compared to C and H cows. Dominant follicle in C (16.00±2.5 mm) and L cows (17.40±2.3 mm) disappeared at 72 h after CIDR removal. However, in H cows, no ovulation has occurred during 7 days post-CIDR removal. Progesterone was not different (p>0.10) among groups, whereas estradiol-17β revealed significant (p<0.01) reduction in H (15.96±2.5 pg/ml) cows compared to C (112.26±26.1 pg/ml) and L (97.49±15.9 pg/ml) cows. Pregnancy rate was higher in L cows (60%) compared with C cows (20%). However, H cows were not artificially inseminated due to non-ovulation. Only a cow of C group has calved one calf, however, 2 of the L cows gave birth of twins and a cow gave single calf.Conclusion:Administration of a low dose (525 IU) of r-hFSH resulted in an optimal size of dominant follicle, normal values of progesterone and estradiol-17β, and 40% twinning rate, howeverusing 1800 IU of r-hFSH, have adverse effects on ovarian follicular dynamics and hormonal profiles with non-pregnancy of dairy cows raised under hot climate.

Highlights

Superovulation of superior cows has been an approach targeting the need for large number of good quality embryos to be transferred [1]
Administration of a low dose (525 IU) of recombinant human follicle stimulating hormone (r-hFSH) resulted in an optimal size of dominant follicle, normal values of progesterone and estradiol-17β, and 40% twinning rate, howeverusing 1800 IU of r-hFSH, have adverse effects on ovarian follicular dynamics and hormonal profiles with non-pregnancy of dairy cows raised under hot climate
We focused on using a new preparation of FSH in cattle, that is, a recombinant human follicle stimulating hormone (r-hFSH) produced in Chinese hamster ovarian cells by recombinant DNA technology [5]

Summary

Introduction

Superovulation of superior cows has been an approach targeting the need for large number of good quality embryos to be transferred [1]. Due to the long acting half-life of equine chorionic gonadotrophin (eCG) in the blood of the stimulated females, adverse effects occur on the embryo quality to be eligible for transfer [2]. Follicle stimulating hormone (FSH) was found to be more efficient to produce better quality embryos, though it requires to be administered in a series of injections due to its short acting half-life of about. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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