Abstract
The aim of this study is to evaluate the biological activity of a crude chitinase extracted from fish offal (scorpion) on the chickpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae) at various concentrations: 3%, 6%, 9%, 12%, 15%, 18%, 21% and 24% (V/V). The biological tests were performed under the typical experimental conditions: temperature and relative humidity at 28°C and 75%, respectively. The results have shown that the concentration of 24% of crude chitinase extracted has 100% of insecticide activity after a contact time of 1 h. The lethal dose (LD50) is estimated for every sample and for a range time from 1 to 72 h. The lower percentage of mortality (10%) is obtained for low values of concentrations and contact time (dose of 3% of crude chitinase and 1 h of contact time). The maximum mortality rate is observed for the high values of two parameters (dose of 24% of crude chitinase and 72 h contact time). However, it is observed that for a higher dose (24%) and for a lower contact time (1 h), the mortality rate increased rapidly. The novelty of this work consists in the use of a new bioinsecticide obtained from offal scorpion fish.
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