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Abstract
Fry availability is an important factor in the cultivation of Sangkuriang catfish. Continuity of fry availability is determined by the number of hatching eggs. The low of hatching rate mainly caused by the Saprolegnia sp. attack. One attempt to overcome the Saprolegnia sp. attack on fish eggs is by using Kecombrang flowers (Nicolia speciosa Horan). Anti-fungal compounds contained in Kecombrang flower was phenol, tannin, alkaloids, and flavonoids. The aim of this research is to find the immersion duration of Sangkuriang catfish eggs in the extract of Kecombrang flower most effectively to prevent the attack of Saprolegnia sp. and increase the egg hatching rate. The method in this study was Completely Randomized Design (RAL) experimental method with five treatments and three replications. The treatments was time of immerse the eggs of Sangkuriang catfish in Kecombrang flower extract at 60 ppm for 10, 15, 20, 25 minutes and without immersion as control. The investigate parameters in this study were Saprolegnia attack rate and hatching rate. Results of the experimental analyzed by F test, and if it was a significance difference then continue with Duncan Multiple Range Test at the 5% level. The results showed that the 20 minutes of immersion duration resulted in the lowest Saprolegnia attack rate about 13.50% and the highest egg hatching rate about 72.50%.
Highlights
Fry availability is an important factor in the cultivation of Sangkuriang catfish
The results showed that the 20 minutes of immersion duration resulted in the lowest Saprolegnia attack rate about 13.50% and the highest egg hatching rate about 72.50%
Jurnal Ilmiah Perikanan dan Kelautan, 6(2): 171
Summary
Bahan Bahan yang digunakan adalah telur ikan lele Sangkuriang sebanyak 3000 butir telur yang terbuahi, 150 butir telur yang tidak terbuahi sebagai media tumbuh jamur, hormon ovaprim, ekstrak bunga Kecombrang, larutan etanol 96% sebagai pelarut dalam pembuatan ekstrak bunga Kecombrang. Tahapan proses maserasi yaitu bunga Kecombrang yang sudah dihaluskan sebanyak 330 gram direndam dalam larutan etanol 96% sebanyak 1 L setiap perendaman. Persiapan awal untuk menumbuhkan jamur Saprolegnia adalah menyiapkan baskom plastik, diisi air sebanyak 2 liter. Telur yang tidak dibuahi dengan ciri-ciri telur berwarna putih dimasukkan ke dalam baskom plastik, kemudian diinkubasi selama sehari agar terinfeksi jamur Saprolegnia. Dan tempat perendaman telur dengan ekstrak bunga Kecombrang, kemudian setiap wadah diisi air sebanyak 2 L. Induk betina yang siap ovulasi kemudian dilakukan proses striping, telur hasil striping di tampung di dalam baskom dan dicampur dengan sperma yang telah diencerkan. Selesai perendaman telur dalam saringan dipindahkan kedalam bak fiber pemeliharaan untuk diinfeksi dengan jamur Saprolegnia sp. Analisis Data Tingkat serangan Saprolegnia sp. dan daya tetas telur dianalisis dengan menggunakan uji F untuk mengetahui pengaruh perlakuan yang diberikan, apabila berpengaruh dilanjutkan dengan uji berganda Duncan pada taraf nyata 5% (10) (Gasperz 1991)
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