Effectiveness evaluation of Trichozone for Trichoderma harzianum and Fulzyme for Bacillus subtillus and Pseudomonas fluorescens in curbing causes of charcoal rot disease on the watermelon plant

Abstract

The results of the study showed the inhibitory effect of the preparation of Trichozone and Fulzyme included the biocontrol agents Trichoderma harzianum and Fulzyme bacterial preparation in Bacillus subtillus and Pseudomonas fluorescens in the laboratory in the growth of colonies of the pathogenic fungus Macrophomina Phaseolina on the PDA agricultural medium, as the two preparations at three concentrations 2.5, 5 and 7.5% achieved in a case of singularity or their combination together a significant inhibition of the pathogenic fungi colony diameter compared to the control treatment, as the interaction between the two factors of Trichozone and Fulzyme achieved the lowest diameter of the growth of that colony as it reached 2.3 cm and a rate of inhibition of 74.44% at the third concentration 7.5%. The results of the study showed the effectiveness of Trichozone and Fulzyme in field to inhibit the charcoal disease on the watermelon plant caused by the pathogenic fungus M. phaseolina, where significant superiority was achieved in treating the combined of the trichozone and Fulzyme and by treating the seeds and watering the seedlings in reducing the severity of infection and reducing the loss in the studied growth characteristics of wet biological weight, dry weight of the root system, plant length and chlorophyll ratio, with an effect average reached 0.17, 1212.1 gm, 3.43 gm, 241.3 cm and 62.8 spad, respectively. Control of pathogen contaminated fungus and untreated achived averages of 0.63, 731.6 gm, 1.81 gm, 145.2 cm and 50.7 spad, respectively. Moreover, the results of the study showed a significant difference in the effect of the same treatment with the difference in the method of application that treatment, the method of using the seed treatment and watering the seedlings superiority on the seed treatment only and for all studied treatments.