Effectiveness Comparison of Bitter Melon Fruit (Momordica charantia L.) Extract with 2% Ketoconazole in Inhibiting Pityrosporum ovale Growth In Vitro

Abstract

Ketoconazole has long been used as an antifungal therapy. One of fungi that causes dandruff is P.ovale. Bitter melon fruit contains antioxidants which can be used as an antifungal. The similarity of antifungal between bitter melon fruit and ketoconazole, made the researchers want to compare the effectiveness of bitter melon fruit extract with 2% ketoconazole in inhibiting P. ovale growth. A laboratory experiment study with Post-test only controls group design, at Microbiology Laboratory, Medical Faculty of Universitas Muhammadiyah Semarang using P.ovale pure isolates. Determination of sample size is done using two proportions formula. Determination of whether or not P.ovale is done macroscopically and microscopically. Sample analysis using the chi-square test. From 30 media given 100% bitter melon extract, 66.7% results did not found in P.ovale growth (20 plates). While 30 media were given 2% ketoconazole, 56.7% did not find P.ovale growth (17 plates). Chi-square test showed p = 0.595 which means there was no difference in effectiveness between giving 100% bitter melon fruit extract and 2% ketoconazole to inhibit P. ovale growth. The effectiveness of 100% bitter melon fruit (Momordica charantia L.) extract equal to 2% ketoconazole in inhibiting P.ovale in vitro growth.