Effectiveness Assessment of a Modified Preservation Solution Containing Thyrotropin or Follitropin Based on Biochemical Analysis in Perfundates and Homogenates of Isolated Porcine Kidneys after Static Cold Storage.

Florian Ryszka,Barbara Dolińska,Aneta Ostróżka-Cieślik

doi:10.3390/ijms22168360

Abstract

In this paper, we assess the nephroprotective effects of thyrotropin and follitropin during ischaemia. The studies were performed in vitro in a model of isolated porcine kidneys stored in Biolasol (FZNP, Biochefa, Sosnowiec, Poland) and modified Biolasol (TSH: 1 µg/L; FSH 1 µg/L). We used the static cold storage method. The study was carried out based on 30 kidneys. The kidneys were placed in 500 mL of preservation solution chilled to 4 °C. The samples for biochemical tests were collected during the first kidney perfusion (after 2 h of storage) and during the second perfusion (after 48 h of storage). The results of ALT, AST, and LDH activities confirm the effectiveness of Biolasol + p-TSH in maintaining the structural integrity of renal cell membranes. Significantly reduced biochemical parameters of kidney function, i.e., creatinine and protein concentrations were also observed after 48 h storage. The protective effect of Biasol + p-TSH is most pronounced after 2 h of storage, suggesting a mild course of damage thereafter. A mild deterioration of renal function was observed after 48 h. The results of our analyses did not show any protective effect of Biolasol + p-FSH on the kidneys during ischaemia.

Highlights

ALT, AST, and lactate dehydrogenase (LDH), was used to diagnose the degree of damage to kidney cells, which intensifies during ischaemia [48,49,50,51]
We investigated the use of Biolasol for kidney storage in this research
We have shown that prolactin (PRL, dose: 1 μg/L) [51,57], lutropin (LH, dose: 0.01 μg/L) [58], and selenium (Se4+, dose: 1 μg/L) [48] have a beneficial protective effect on the kidneys

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. One of the challenges in modern transplant technology is the increasing volume of organ donations. Owing to advances in transplant medicine, it has become increasingly possible to successfully transplant organs from older donors, who are often burdened with comorbidities. Searching for solutions that will improve the quality of marginal organs is considered a priority. The methods of storage before transplantation are of great importance for ensuring the good functional quality of grafts

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