Effectiveness and clinical applications of real-time fluorescence-based quantitative PCR system for the detection of Clostridium perfringens

Abstract

2cfu/ml pure C. perfringens, or 9 cfu/reaction. The procedure was stable, repeatable and fast, taking only 3 h in total. Detection results of 500 wound secretions using this PCR method were consistent with those obtained using conventional culture detection methods, demonstrating for the first time that this fluorescence-based quantitative PCR system is effective, accurate and applicable on a large sample scale under clinical conditions. We have thus provided a highly specific, sensitive, fast, and accurate fluorescence-based quantitative PCR technique that can quickly diagnose the early stages of gas gangrene. This may be especially valuable in batch testing, such as in the event an earthquake or other emergency.