Effective use of a disposable sperm counting chamber for improving the preparation of infected sperm samples

Abstract

ObjectiveWe often use the Makler counting chamber to count sperm in both vitro fertilization (IVF) and intrauterine insemination (IUI) treatments. However, the Makler must be washed before reuse. Moreover, when dealing with infected sperm samples, the transmission of infectious diseases is possible. To improve the preparation of infected sperm samples and limit the transmission of infectious diseases, we examined the viability of disposable sperm counting chambers (hereafter, Dispo).DesignProspective study.Materials and MethodsWe analyzed C-ChipDHC-S01 (INCYTO), Cell-VU (Millennium Sciences), MC-CV-1010-G and MC-CV-1020-G (CellVision) as Dispo. We then examined motile and total number of sperm in each counting chamber, including Makler (Sefi-Medical Instruments Ltd.), after treatment. The examinations were carried out in triplicate and the means were calculated.ResultsComparing Makler and C-ChipDHC-S01, the number of motile sperm (106/ml) and the total sperm count were 17.5±5.3 and 15.6±4.6, and 54.8±13.6 and 43.2±11.1, respectively. Comparing Makler and Cell-VU, the number of motile sperm (106/ml) and the total sperm count were 20.3±4.5 and 14.5±3.6, and 30.2±5.0 and 20.5±3.8, respectively. Comparing Makler and MC-CV-1010-G and MC-CV-1020-G, the numbers of motile sperm (106/ml) were 17.4±6.4, 14±5.2, and 9.7±3.3, respectively, and the total sperm counts were 28.0±7.4, 26.0±6.7, and 20.2±4.6, respectively. No significant differences were observed in either motile sperm count or total sperm count between Makler and each Dispo.ConclusionBoth Dispo and Makler can be used for accurately counting sperm. Notably, the interval of the grid lines is identical for both MC-CV-1010-G and Makler, and the sperm counts obtained using MC-CV-1010-G and Makler were nearly equal. Therefore, we suggest using MC-CV-1010-G for infected sperm samples because it is disposable, which helps to eliminate the possibility of transmitting infectious diseases. ObjectiveWe often use the Makler counting chamber to count sperm in both vitro fertilization (IVF) and intrauterine insemination (IUI) treatments. However, the Makler must be washed before reuse. Moreover, when dealing with infected sperm samples, the transmission of infectious diseases is possible. To improve the preparation of infected sperm samples and limit the transmission of infectious diseases, we examined the viability of disposable sperm counting chambers (hereafter, Dispo). We often use the Makler counting chamber to count sperm in both vitro fertilization (IVF) and intrauterine insemination (IUI) treatments. However, the Makler must be washed before reuse. Moreover, when dealing with infected sperm samples, the transmission of infectious diseases is possible. To improve the preparation of infected sperm samples and limit the transmission of infectious diseases, we examined the viability of disposable sperm counting chambers (hereafter, Dispo). DesignProspective study. Prospective study. Materials and MethodsWe analyzed C-ChipDHC-S01 (INCYTO), Cell-VU (Millennium Sciences), MC-CV-1010-G and MC-CV-1020-G (CellVision) as Dispo. We then examined motile and total number of sperm in each counting chamber, including Makler (Sefi-Medical Instruments Ltd.), after treatment. The examinations were carried out in triplicate and the means were calculated. We analyzed C-ChipDHC-S01 (INCYTO), Cell-VU (Millennium Sciences), MC-CV-1010-G and MC-CV-1020-G (CellVision) as Dispo. We then examined motile and total number of sperm in each counting chamber, including Makler (Sefi-Medical Instruments Ltd.), after treatment. The examinations were carried out in triplicate and the means were calculated. ResultsComparing Makler and C-ChipDHC-S01, the number of motile sperm (106/ml) and the total sperm count were 17.5±5.3 and 15.6±4.6, and 54.8±13.6 and 43.2±11.1, respectively. Comparing Makler and Cell-VU, the number of motile sperm (106/ml) and the total sperm count were 20.3±4.5 and 14.5±3.6, and 30.2±5.0 and 20.5±3.8, respectively. Comparing Makler and MC-CV-1010-G and MC-CV-1020-G, the numbers of motile sperm (106/ml) were 17.4±6.4, 14±5.2, and 9.7±3.3, respectively, and the total sperm counts were 28.0±7.4, 26.0±6.7, and 20.2±4.6, respectively. No significant differences were observed in either motile sperm count or total sperm count between Makler and each Dispo. Comparing Makler and C-ChipDHC-S01, the number of motile sperm (106/ml) and the total sperm count were 17.5±5.3 and 15.6±4.6, and 54.8±13.6 and 43.2±11.1, respectively. Comparing Makler and Cell-VU, the number of motile sperm (106/ml) and the total sperm count were 20.3±4.5 and 14.5±3.6, and 30.2±5.0 and 20.5±3.8, respectively. Comparing Makler and MC-CV-1010-G and MC-CV-1020-G, the numbers of motile sperm (106/ml) were 17.4±6.4, 14±5.2, and 9.7±3.3, respectively, and the total sperm counts were 28.0±7.4, 26.0±6.7, and 20.2±4.6, respectively. No significant differences were observed in either motile sperm count or total sperm count between Makler and each Dispo. ConclusionBoth Dispo and Makler can be used for accurately counting sperm. Notably, the interval of the grid lines is identical for both MC-CV-1010-G and Makler, and the sperm counts obtained using MC-CV-1010-G and Makler were nearly equal. Therefore, we suggest using MC-CV-1010-G for infected sperm samples because it is disposable, which helps to eliminate the possibility of transmitting infectious diseases. Both Dispo and Makler can be used for accurately counting sperm. Notably, the interval of the grid lines is identical for both MC-CV-1010-G and Makler, and the sperm counts obtained using MC-CV-1010-G and Makler were nearly equal. Therefore, we suggest using MC-CV-1010-G for infected sperm samples because it is disposable, which helps to eliminate the possibility of transmitting infectious diseases.