Abstract
The accuracy of nucleic acid extraction and diagnosis depends on the sealing performance of the pipetting device. The rubber seal ring at the front end of the pipette shaft is a critical component for ensuring the sealing performance of the pipetting device. In this study, an accurate prediction and analysis were made on the fatigue life of the pipette shaft seal ring. ABAQUS was used to simulate the assembly process of pipette shaft seal ring and disposable tip, and the dangerous cross-section segments on the seal rings were preliminarily identified according to a stress nephogram. Based on the continuum damage mechanics theory, the fatigue life prediction model was built where the effective strain was taken as a damage parameter, and the fatigue life of the dangerous cross-section of the pipette shaft seal ring was calculated. By observing failed seal rings in actual use, it was found that the worn positions of the failed seal rings were the same as the sites of the shortest-lived node segments, thus verifying the accuracy of fatigue life prediction model and fatigue life analysis.
Highlights
As a consequence, the seal ring at the front end of the pipette shaft has to be inserted into and pulled out of tips for tens of thousands of times
There are very few reports on fatigue life prediction of pipette shaft seal rings susceptible to large deformation caused by shear and extrusion loads
In view of the long period and high cost of model experiment under actual physical conditions, this study is based on the theory of continuum damage mechanics, combined with the special working conditions of frequent assembly of sleeve seals, and established the equivalent effect of sleeve seals in the assembly process
Summary
The seal ring at the front end of the pipette shaft has to be inserted into and pulled out of tips for tens of thousands of times. [12,13,14], according to the particle distribution and crack expansion characteristics in the sample, propose a method for predicting the life of filled elastomers based on particle distribution and J-integral evaluation. In view of the long period and high cost of model experiment under actual physical conditions, this study is based on the theory of continuum damage mechanics, combined with the special working conditions of frequent assembly of sleeve seals, and established the equivalent effect of sleeve seals in the assembly process. The fatigue life prediction model with the variable range as the damage parameter is used to calculate the fatigue life of the dangerous section element on the sleeve sealing ring. The accuracy of pipette nucleic acid extraction, and provided theoretical guidance and design basis for the structure optimization of the pipette
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