Abstract
BackgroundDue to a multitude of industrial applications of keratinolytic proteases, their demands are increasing. The present investigation studied the production and monitoring of the most possible multi-functional applications of YNDH thermoalkaline keratin-degrading enzyme. ResultsThis work is considered the first that reported YNDH strain closely related to Laceyella sacchari strain; YNDH is a producer of protease/keratinase enzyme and able to degrade natural keratin such as feathers, wool, human hairs, and nails. Experimental design Plackett-Burman (PBD) was applied to evaluate culture conditions affecting the production of thermoalkaline protease/keratinase. Afterwards, Box-Behnken design (BBD) was applied to find out the optimum level of significant variables namely, NH4Cl, yeast extract, and NaNO3 with a predicted activity of 1324.7 U/ml. Accordingly, the following medium composition and parameters were calculated to be optimum (%w/v): NH4Cl, 0.08; feather, 1; yeast extract, 0.04; MgSO4.7H2O, 0.02; NaNO3, 0.016; KH2PO4, 0.01; K2HPO4, 0.01; pH, 8; inoculum size; 5%, cultivation temperature (Temp.) 45 °C and incubation time 48 h. The studied enzyme can degrade keratin-azure, remove proteinaceous materials, and is able to remove hairs from goat hides. These interesting characteristics make this enzyme a good candidate in many applications especially in detergent (Det.), in leather industries, and in pharmaceuticals particularly in nail treatment. ConclusionThe promising properties of the newly keratin-degrading protease enzyme from Laceyella sacchari strain YNDH would underpin its efficient exploitation in several industries to cope with the demands of worldwide enzyme markets.
Highlights
Due to a multitude of industrial applications of keratinolytic proteases, their demands are increasing
The primary purpose of this research is to assess the circumstances of culture that affect the output of thermoalkaline protease with keratinolytic activity by locally isolated Laceyella sacchari YNDH and the possible application of this enzyme
This study is considered the first work to report that Laceyella sacchari YNDH is a producer of protease/keratinase enzyme, so this strain is distinct in concern its dual activities
Summary
Due to a multitude of industrial applications of keratinolytic proteases, their demands are increasing. Causes the loss of a certain number of amino acids such as methionine, lysine, and tryptophan Conventional disposal methods such as incineration of feathers have ecological disadvantages. The primary purpose of this research is to assess the circumstances of culture that affect the output of thermoalkaline protease with keratinolytic activity by locally isolated Laceyella sacchari YNDH and the possible application of this enzyme. In this way, the theme chosen for the application research can be exploited in many industries such as the leather industry, feeding, medical, and others
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