Effective identification of self-incompatibility in Brassica oleracea var. italica with fluorescent observation.

Abstract

The self-incompatibility (SI) in advanced inbred lines of Brassica oleracea var.italica Plenck was identified with the compatible index method and fluoroscope observation. The compatible index was tested with bud and flowering pollination. Most pollen grains of selfing in self-incompatible at bud stage and in self-compatible individuals at both bud and floral stages could germinate normally, and the pollen tubes penetrated into the stigmas and finally succeeded in reaching to the ovary to accomplish fertilization. While selfing at anthesis, few pollen grains in self-incompatible individuals could germinate in the stigma whose surface produced serious callous reaction, and few germinated pollen grains could not grow normally and fail to penetrate into the stigma. The fluoroscope observation, being in high accordance with the results of the compatible index, was convenient and accurate without effecting by surroundings, and could be applied in self-incompatibility identification effectively to accelerate the process of self-incompatibility utilization in B.oleracea var.italica Plenck breeding program.