Abstract
Fermentation profiling is a common tool to identify Lactobacillus paracasei from other related members of Lactobacillus genus. Because of the proximate biochemical characteristics, identification based on the fermentation pattern within L. casei group (L. casei, L. paracasei and L. rahmnosus) has considerable limitation. On the other hand, members of L. casei group are genetically very closely related and sequencing of signature region is the most reliable and rapid method of differentiation. In this study, instead of sequencing entire 16S or 23S ribosomal RNA gene, we have sequenced 16s-23s rRNA intergenic spacer region and compared them across the members of L. casei group. We also compiled thirteen signature sequences within 16s-23s ISR rRNA gene of L. casei group members. Our isolated strain (L. paracasei ssp. paracasei-1) resembled 100% and 99% similarity when compared with 16s-23s rRNA intergenic spacer region of L. paracasei and L. casei respectively. Our study summarizes that sequencing of short 16s-23s rRNA intergenic spacer region carries great significance in identification of closely related probiotic bacteria such as the members of L. casei group. The findings of this research could be very much helpful for food and pharmaceutical industries who are dealing with probiotic bacteria.
Highlights
The genus Lactobacillus is subject to continual taxonomic changes
Coudeyras et al (2008) classified L. casei into four group members, we have considered L. casei with three group members including L. casei, L. paracasei and L. rhamnosus based on the latest phylogenic structure revealed by Salvetti et al (2012)
We obtained PCR amplification of 16s-23s intergenic spacer region (ISR) rRNA gene based on which we reported the isolate as Lactobacillus paracasei ssp. paracasei-1
Summary
The genus Lactobacillus is subject to continual taxonomic changes. It is often thorny to use classical microbiological methods to identify lactic acid bacteria even to genus level because of their similar nutritional and growth requirements (Dubernet et al, 2002). Coudeyras et al (2008) classified L. casei into four group members, we have considered L. casei with three group members including L. casei, L. paracasei and L. rhamnosus based on the latest phylogenic structure revealed by Salvetti et al (2012) Considering these identification enigma, use of a diagnostic marker specific to the probiotic strain of interest carries significant importance. In this study we have compared the sequences of 16s-23s ISR region of L. casei group along with the identification of L. paracasei ssp. Paracasei-1 (Islam et al, 2012; Honi et al, 2013) and comparing the sequenced results across the already reported 16s-23s ISR region sequences of L. casei group members, in order to determine signature sequences, are the main focus of this research Sequencing of the 16s-23s ISR region of previously identified L. paracasei ssp. paracasei-1 (Islam et al, 2012; Honi et al, 2013) and comparing the sequenced results across the already reported 16s-23s ISR region sequences of L. casei group members, in order to determine signature sequences, are the main focus of this research
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