Effective Construction of a High-Capacity Boronic Acid Layer on a Quartz Crystal Microbalance Chip for High-Density Antibody Immobilization.

Pei-Heng Lin,Yaw-Kuen Li,Bor-Ran Li,Kuang-Po Chen,Sheng-Cih Huang

doi:10.3390/s19010028

Pei-Heng Lin, Yaw-Kuen Li + Show 3 more

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https://doi.org/10.3390/s19010028

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Abstract

Boronic acids (BAs) provide strong potential in orientation immobilization of antibody and the modification method is crucial for efficiency optimization. A highly effective method has been developed for rapid antibody immobilization on gold electrodes through the electrodeposition of a BA–containing linker in this study. Aniline-based BA forms a condense layer while antibody could automatically immobilize on the surface of the electrode. Compare to traditional self-assembled monolayer method, the electrodeposition process dramatically reduces the modification time from days to seconds. It also enhances the immobilized efficiency from 95 to 408 (ng/cm2) with a strong preference being exhibited for shorter aniline-based linkers.

Highlights

Biosensors have attracted a great deal of attention because of their potential to be developed into tools capable of rapidly diagnosing various diseases [1]
The suitability of quartz crystal microbalance (QCM)-based biosensors for immunosensors is favorable in fields such as drug discovery [9], molecular interaction [10], and clinical diagnosis [11]
QCM was employed to evaluate the immobilization efficiency of these compounds and compare Ab loading capacities. 3-Aminophenylboronic acid is first conjugated with chains of varying lengths, such as aminobutyric acid and lysine, and aniline is added to the Boronic acids (BAs)

Summary

Introduction

Biosensors have attracted a great deal of attention because of their potential to be developed into tools capable of rapidly diagnosing various diseases [1]. The QCM chip has an exceptionally robust structure and ionic-strength-independence characteristics and enables the measurement of changes in mass at the molecular level, distinguishing the QCM system as one of the most attractive biosensors [8]. The suitability of quartz crystal microbalance (QCM)-based biosensors for immunosensors is favorable in fields such as drug discovery [9], molecular interaction [10], and clinical diagnosis [11]. Because of their simplicity and rapid, label-free detection This biosensing system can measure the variation of mass change to the nanogram scale with high precision [12]. QCM responds to the binding of analytes to the sensing layer [13] by changing frequency, which usually immobilizes the bioreceptors capable of capturing certain targets through site-specific recognition. Covalent bonds are Sensors 2019, 19, 28; doi:10.3390/s19010028 www.mdpi.com/journal/sensors

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