Abstract
BackgroundThe aim of this study was to evaluate the effect of autogenous tooth bone as a graft material for regeneration of bone in vertical bony defects of the minipigs.Material and MethodsSix minipigs were used in this study. Four molars were extracted in the right mandibular dentition and sent to the Korea Tooth Bank for fabrication of autogenous tooth bone. Ten days later, each extraction site was implanted with MS Implant Narrow Ridge 3.0x10mm fixture (Osstem, Seoul, Korea) after standardized 2mm-sized artificial vertical bony defect formation. Pineappleshaped Root-On type autogenous tooth bones were applied to the vertical defects around the neck area of the posterior three fixtures and the fore-most one was not applied with autogenous bone as a control group. Each minipig was sacrificed at 4, 8, 12 weeks after fixture installation and examined radiologically and histologically. Histological evaluation was done under light microscope with Villanueva osteochrome bone staining with semi-quantitative histomorphometric study. Percentage of new bone over total area (NBF) and bone to implant contact (BIC) ratio were evaluated using digital software for area calculation.ResultNBF were 48.15 ± 18.02%, 45.50 ± 28.37%, and 77.13 ± 15.30% in 4, 8, and 12 weeks, respectively for experimental groups. The control group showed 37.00 ± 11.53%, 32.25 ± 26.99%, and 1.33 ± 2.31% in 4,8,12 weeks, respectively. BIC ratio were 53.08 ± 19.82%, 45.00 ± 28.37%, and 75.13 ± 16.55% in 4,8,12 weeks, respectively. Those for the control groups were 38.33 ± 6.43%, 33.50 ± 29.51 %, and 1.33 ± 2.31% in 4, 8, 12 weeks, respectively.ConclusionAutogenous tooth bone showed higher score than control group in NBF and BIC in all the data encompassing 4,8,12 weeks specimens, but statistically significant only 12 weeks data in both NBF and BIC.
Highlights
The aim of this study was to evaluate the effect of autogenous tooth bone as a graft material for regeneration of bone in vertical bony defects of the minipigs
New bone formation was observed from the margin of the defect toward the center of the defect (Figure 3)
The control group showed an aspect of new bone formation, the degree was lower compared to the experimental group (Figure 4)
Summary
The aim of this study was to evaluate the effect of autogenous tooth bone as a graft material for regeneration of bone in vertical bony defects of the minipigs. Bone grafts are being made using materials extracted from autogenous teeth. The components of tooth bone, which is a graft material made by using teeth, have already been experimentally verified in many studies and papers [4,5,6,7,8,9]. Those studies are different from the present study, as most of them are in regard to tooth bone made from other persons’ teeth already extracted after removing all organic components for immunological safety and safety against infection transmission. The present study approached the effectiveness of autogenous tooth bone grafts that have the same benefits as autogenous bone grafts in that rejection of transplant can be fundamentally blocked because autogenous tooth bone, immunologically speaking, has the same origin as autogenous bone, so that the possibility of the transmission of a source of infection between different persons or species can be fundamentally blocked
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