Abstract

Abstract Inoculation with Rhizobium meliloti and nitrogen fertilization on alfalfa increasedthe plant density, forage fresh yield and the protein content, and decreased the crude fibrecontent of alfalfa. Keywords: alfalfa, forage yield, nitrogen fixation, Rhizobium . Introduction Alfalfa is the most important cultivated forage legume in the Sudan, where it is grown underirrigation usually without fertilizers. Elsewhere the effect of nitrogen fertilization has varied:for example Giroux (1981) found that 40kg N/ha before sowing significantly increased theyield on a silty clay loam, but not on clay. The effect of Rhizobium inoculation also varies(Kots et al. 1995; Provorov and Tikhonovich 2003). This study concerned the effect of Rhizobium inoculation on growth, symbiosis, quality and forage yield, and the performanceof alfalfa under increasing doses of nitrogen. Materials and methods Rhizobium meliloti strain ENRRI A12 was supplied by the National Centre for Research,Khartoum. Broth culture (400 ml of 1 × 10

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