Abstract
Z acid (ZA) is a bisphosphonate that is used to prevent skeletal fractures in patients with cancer, as well as for treating osteoporosis. Complete mechanism of action of ZA is not understood, and their actions on the osteoblasts are confusing. Epigenetic changes such as DNA methylation are regulatory mechanisms that control stem cell differentiation. In this situation, ZA induces osteoblastic differentiation in MSC, but regulatory mechanisms are unknown. Gene specific methylation of RUNX2, OSX, DLX5 and BSP genes and global methylation status were evaluated by methylation specific PCR (MSP) and specific antibody, respectively. Also, the effect of ZA on the BSP gene expression was evaluated by RT-PCR.
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