Effect of Zingiber Officinale (Ginger Rhizomes) Hydroethanolic Extract on Hyoscine-Induced Memory Impairment in Adult Male Rats

Abstract

Background: The spice Zingiber officinale or ginger possesses antioxidant activity andneuroprotective effects. In this study we hypothesized that treatment with hydroethanolicextract of ginger (50, 100 and 200 mg/kg, p.o) would effect on the hyoscine-induced memoryimpairment in rats.Methods: In this experimental study 64 male Wistar rats were divided into eight groups (8 ratsin each group): normal saline, hyoscine (1 mg/kg), ginger extract (50, 100 and 200 mg/kg),or hyoscine (1 mg/kg) plus ginger extract (50, 100 and 200 mg/kg). Memory impairment wasinduced by a single injection of hyoscine (1 mg/kg, i.p). Cognitive functions were evaluatedusing passive avoidance learning (PAL) task. Retention test was carried out 24 h after training,and the latency of entering the dark compartment [step-through latency (STL)] and the totaltime in the dark compartment (TDC) were recorded. All statistical analysis was carried out at5% level of significance using SPSS version 21. The data were analyzed by ANOVA followedby Tukey’s test.Results: The time latency in hyoscine-treated group was lower than control (133.87±14.60vs. 242.12±10.58; p<0.001, respectively). Treatment of the animals by 100 and 200 mg/kg ofginger extract before the training trial increased the time latency at 24 h after the training trial(277±4.67 and 280.37±7.68; p<0.01, respectively). Administration of both 100 and 200 mg/kgdoses of the extract in hyoscine received animal groups before retention trials also increasedthe time latency than the hyoscine-treated groups (247.37±7.62 and 271.87±9.11; p<0.001,respectively).Conclusion: The results revealed that the ginger hydroethanolic extract attenuated hyoscineinducedmemory impairment.