Abstract
Abstract Zinc as an essential micronutrient for plant metabolism was prepared as zinc oxide nanoparticles (ZnO-NPs), where their structure and morphology were investigated by X-ray diffraction and transmission electron microscope. A suspension solution of ZnO-NPs was used as a foliar fertilizer for common bean with the concentration 0, 10, 20, 30 and 40 ppm, during two successive seasons 2016 and 2017, to study there on the production and the quality of common bean. Also, the Genotoxic effect of ZnO-NPs on genomic DNA of common bean by RAPD-PCR and on the expression of some genes encoding proteins by SDS-PAGE was studied. It was found that the best values of common bean plant criteria i.e. the number of branches and leaves per plant, fresh and dry weight of branches and leaves, as well as the highest yield of seeds (2.41 and 2.48 ton/ha during 2016 & 2017, respectively) were obtained with 30 ppm of ZnO-NPs. The RAPD analysis showed that the ZnO-NPs (20 and 30 ppm) effect on the plant genome and on the expression of some genes encoding proteins. Also, the SDS-PAG analysis represented those unique proteins at the molecular weight 78 KDa appeared in the plant treated by 30ppm ZnO-NPs these proteins may play an important role in enhancement the agro-morphological criteria of the plant.
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