Effect of zinc nanoaquacitrate on the biochemical and productive parameters of the organism of rabbits

О V Boiko,Y V Lesyk,І І Kovalchuk,B V Gutyj,О F Honchar

doi:10.15421/022036

Abstract

Zinc is an essential microelement for the organism of animals, and its deficiency reduces the synthesis and degradation of proteins, lipids, carbohydrates and nucleic acids, and also decreases the metabolism of vital microelements. The objective of the study was determining the effect of various amounts of zinc nanoaquacitrate on the biochemical parameters of blood and productivity of the organism of rabbits after weaning on 50th to 86th days of life. Compared with the control group, intake of zinc nanoaquacitrate by the animals of the experimental groups exhibited dose-dependent effect on separate biochemical parameters which characterize the processes of lipid and mineral metabolisms, which manifested in the increase in their content in the blood on the 12th, 24th and 36th days of the experiment. Watering rabbits with zinc citrate in the dose of Zn 0.25 mg/kg after weaning determined its physiological effect on the biochemical parameters of their organism, expressed in the most statistically reliable changes compared with other tested amounts, particularly a higher level of the total protein, increase in the activities of glutathione reductase, alanine aminotransferase, catalase and alkaline phosphatase and decrease in the levels of cholesterol, lipid hydroperoxides, compared with the control, which may indicate the positive effect of the applied additives on certain biochemical parameters of the rabbits’ organism. Additional use of zinc citrate in the dose of 0.50 mg/kg of body weight was manifested in their blood by significant increase in the activities of catalase and glutathione reductase, lower level of lipid hydroperoxides and increase in the organism’s growth parameter by 3.1% in the final period of the study. Watering zinc citrate in the amount of 0.75 mg/kg of body weight provoked probable decrease in the level of cholesterol and triacylglycerols and increases in catalase and glutathione reductase, increase in activity of alanine aminotrasferase and decrease in lipid hydroperoxides, which determined the stimulating effect on the growth of the organism with 8.4% higher total gain of body weight after 36 days of the experiment. From a general perspective, the research indicates that intake of lowest amount of zinc citrate was characterized by the greatest effect on the biochemical blood parameters, whereas the use of greater amounts of organic compound of zinc showed positive effect on the processes of growth and development of the organism of rabbits after weaning. Of practical importance is the study of the effect of watering zinc nanoaquacitrate in the physiologically substantiated amounts on biochemical blood parameters and growth and survivability of the offspring and organism of the mother rabbit in the period of lactation.

Highlights

In the diet of rabbits, microelements are important component of a number of metabolic processes and physiological functions of their organism (Darmohray et al, 2019)
The content of total protein in blood of rabbits of the experimental groups compared with the control in the preparation period did not undergo any significant changes (Table 1), whereas in the blood of rabbits of the I experimental group the level of total protein was respectively higher by 14.5% and 14.0% (Р < 0.05) on the 12th and 36th days of the study compared with the control
This manifested in reliable increase in the activity of alanine aminotransferase in the blood of animals of the I experimental group by 34.7% (Р < 0.05) on the 24th day and by 19.6% in the ІІІ group on the 36th day of the experiment, compared with the control

Summary

Introduction

In the diet of rabbits, microelements are important component of a number of metabolic processes and physiological functions of their organism (Darmohray et al, 2019). In the organism of animals, zinc is a constituent of over 300 enzymes which participate in synthesis and degradation of proteins, lipids, carbohydrates and nucleic acids, and other microelements involved in metabolism (Saleh et al, 2018). It performs the function of co-factors of enzymes (Grosskopf et al, 2017). In physiological amounts in the organisms of humans and animals, zinc affects the regulation of acid-base homeostasis and activation of metabolism It is a necessary component of superoxide dismutase enzyme, which performs a vital function in the system of antioxidant protection of the organism (Ivanišinová et al, 2016). Higher levels of zinc in the diet than are necessary for the fulfillment of the needs of the rabbits’ organism may cause toxicoses in young animals, death of offspring and pregnant animals and have negative impact on the environment, the use of novel compounds of microelements in nano-sized amounts is promising (Amen & Daraji, 2011; Raguvaran et al, 2015)

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