Abstract

The modulation of α-mannosidase enzyme activity has been investigated. Two studies have been conducted: (a) the effect of zinc ions over enzyme activity has been evaluated; if stored for 20h in acetic acid/acetate buffer, pH 5, supplemented with 1mM ZnSO4, the enzyme displayed higher catalytic capacity than the enzyme stored in the same buffer without zinc ions; (b) the effect of EDTA on enzyme activity has been investigated, in presence of 10 mM EDTA in acetic acid/acetate buffer, pH=5; EDTA inhibited the enzyme, even without pre-incubation.

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