Abstract
The effects of the aqueous extract of a popular variety zarda (processed tobacco used in betel) on the root meristems of Allium cepa Linn. were studied. While the higher concentrations (1.5% and 1%) of the extract were highly mitostatic, lower concentrations (0.75%, 0.5% and 0.25%) produced various types of chromosomal damage. The most conspicuous features were the stretching and fracture in the centromeric regions which became evident at metaphase. Various other abnormalities like clumped metaphases and anaphases, bridge connections at anaphase and pulverization of chromosomes leading to nuclear disintegration were also met with.
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