Abstract
Introduction: People worldwide drink Zamzam water either medicinally or religiously. However, the effect of zamzam water extract of CZ in breast cancer cells remains unclear. Objective: To evaluate the zamzam water extract of CZ effect on cell apoptosis, p53 and Bcl2 protein expression in T47 D cells line culture. Methods: In the control group design, 16 Wells which was filled with T47 D cell line culture in the density of 5 × 10 4 /100 μl, were assigned into 4 groups. Control group (C-G), were only filled with T47D cell line culture; Zamzam 50 (ZZ-50); Zamzam 100 (ZZ-100), and Zamzam 500 (ZZ-500) groups, were filled with T47D cell line cultures and 50, 100, and 500 μg/ml zamzam water extract of CZ respectively, and then incubated for 48 hours. The number of apoptotic cell was counted by flow cytometry, while p53 and Bcl2 measured by immunohistochemistry staining method. Results: Mann Whitney analysis indicated that the percentage of apoptotic cell and p53 protein expression in ZZ-100 was significant higher compared to that of C-G (p < 0.05). In contrary the percentage number of Bcl2 protein expression in ZZ100-G was significantly lower when compared to that of C-G (p < 0.05). Conclusion: Treatment of zamzam water extract of CZ on T47D cell line cultured was capable of increasing the number of apoptotic cells, p53 protein expression, but decreased Bcl2 protein expression in T47D cell line culture. However the numbers of apoptotic cells were less than 10%.
Highlights
People worldwide drink Zamzam water either medicinally or religiously
Post test only control group design, 12 wells which was filled with culture of T47 D cell line in the density of 5 x 104/100 μl were assigned into 4 groups, three wells of each consist of: Control group (C-G), was only filled with T47D cell line culture; Zamzam 50 (ZZ50-G), Zamzam 100 (ZZ100-G), and Zamzam 500 (ZZ500-G) groups, were filled with T47D cell line culture and treated with 50, 100, and 500 μg/ ml Curcuma zedoaria (CZ) diluted in Zamzam water respectively
Apoptosis T47D cells following the treatment with Curcuma zedoaria was dissolved in zamzam water was analyzed by flow cytometry FITC apoptosis detection kit with Propidium Iodide (Sigma-Aldrich)
Summary
The effect of zamzam water extract of CZ in breast cancer cells remains unclear. Objective: To evaluate the zamzam water extract of CZ effect on cell apoptosis, p53 and Bcl[2] protein expression in T47 D cells line culture. Conclusion: Treatment of zamzam water extract of CZ on T47D cell line cultured was capable of increasing the number of apoptotic cells, p53 protein expression, but decreased Bcl[2] protein expression in T47D cell line culture. The mortality rate of breast cancer had reduced to 24% on women with Estrogen-Progesterone Receptor positive cancer disease[4]. The decrease in breast cancer mortality rate was attributable to the combination of early detection with screening programs and the efficacious adjuvant systemic therapy[5]. Invitro studies provide evidence that flavonoids may be associated with cell proliferation inhibition, adhesion, and invasion, inducing cell differentiation, cell cycle arrest, and apoptosis[7]
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