Effect of yeast extract, peptone, and certain nitrogen compounds on sporulation of Saccharomyces cerevisiae.

Abstract

Aeration of cells for 24 hrs. previous to placing them in 0.1% sodium acetate solution diminished sporulation, but this decrease was overcome by the addition of 0.1% yeast extract to the acetate solution. Cells starved by growth on Czapek solution agar +0.03% peptone formed very few ascospores in acetate solution. The addition of yeast extract or peptone in low content to the acetate solution increased the yields. However, the cells did not form as many ascospores as well-nourished cells in acetate solution.