Abstract
The growth of C. albicans is influenced by glucose intake. Xylitol is commonly used as sugar substitute. Reported effective concentrations of xylitol in reducing C. albicans growth in vitro were varied, 1%, 5%, and 10%. Objectives: Investigate the effect of different concentration and duration of xylitol exposure in inhibiting C. albicans growth in vitro. Method: Identification of C. albicans from oral swab of a male candidiasis patient was conducted using CHROMagar, confirmed by germ tube test. C. albicans suspension (108 cells/μl) were inoculated in SDB contained 1%, 5%, 10% xylitol, and without xylitol (as control), for 3 and 7 days, then incubated in 37oC on SDA and counted for their CFU after 48 hours. The C. albicans ATCC 10231 strain was used as a comparison. Results: After 3 days, increased concentration of xylitol (1%, 5%, 10%) lead to decrease growth of C. albicans, both the ATCC 10231 (125%; 51%; 14% respectively) and the clinical isolate (103%; 81%; 42%), p = 0.044. Significant lower growth of C. albicans compared to control were only seen in those exposed to 10% xylitol (p = 0.024). After 7 days, exposure of 1%, 5%, 10% xylitol did not significantly affect the growth of C. albicans (p = 0.396). Conclusion: The growth of C. albicans could be inhibited by 10% xylitol for 3 days. DOI: 10.14693/jdi.v16i1.12
Highlights
Candidiasis is the most common fungal infection of human and mostly caused by Candida albicans.[1,2,3] In the last two decades, infection cases due to Candida species has increased significantly.[4]
The mean results of extrapolation that have been achieved from C. albicans clinical isolate was 9 x 108
Colony Forming Unit (CFU)/ml and from the ATCC 10231 strain was 1,5 x 108 CFU/ml. This score will be compared to the CFU of those exposed to xylitol with various concentrations (1%, 5%, 10%) and different duration (3 or 7 days) to get the percentage of C. albicans growth
Summary
Candidiasis is the most common fungal infection of human and mostly caused by Candida albicans.[1,2,3] In the last two decades, infection cases due to Candida species has increased significantly.[4]. If the balance of the normal flora is disrupted or the immune defenses are compromised, Candida species often become pathogenic.[7,8]
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