Abstract
Xylanase production by the alkalophilic Bacillus pumilus was improved under submerged fermentation when commercial Birchwood xylan was used as the substrate. Initially the medium containing commercial Birchwood xylan 20gL -1 at pH 8.5 was used. When the commercial Birchwood xylan concentration was 5gL -1 highestxylanase activity [61.3(±0.96) UmL -1 ] was obtained at 24 h. The media substituted with raw materials of local carbon sources and considerable amount of xylanase was produced in the media containing corn cob[24.9(±0.99) UmL -1 ], corn hull [18.2(±0.92) UmL -1 ] and rice straw [21.5(±0.86) UmL -1 ] and lowest amount of activity was obtained with rice bran [4.7(±0.98) UmL -1 ]. Xylan was extracted from local carbon source such as corncob, corn hull and rice straw and these were used as the carbon source in the media and Birchwood xylan was used as the control. The highest xylanase activity was obtained in the medium with Birchwood xylan [(62.6(±0.89) UmL -1 )] than in the media which contained the xylan (5gL -1 ) extracted from corncob [29.8(±0.97) UmL -1 ] corn hull [20.2(±0.99) UmL -1 ] and rice straw [17.6(±0.98) UmL -1 ]. Due to the amount of xylose present in the xylan, the highest activity was obtained with Birch wood xylan. Based on this study the xylanase production from B.pumilus was better with commercial Birchwood xylan than that extracted from different local sources. Ving. Journal of Science 2012, Vol. 11, No. 1, 12-18
Highlights
Xylanase has attached special attention due to its potential applications in many processing industries (Lemoset al., 2001; Wong et al, 2000)
In this study xylanase producing bacterial strain Bacillus pumilus isolated from corncob decaying soil was used for enzyme production (Jesuthasanet al., 2010)
In the following experiments locally available xylan sources such as corncob, corn hull, rice straw and rice bran powders were used as carbon sources for xylanase production
Summary
Xylanase has attached special attention due to its potential applications in many processing industries (Lemoset al., 2001; Wong et al, 2000). The use of purified xylan as a substrate for bioconversion into xylanase increases the cost of enzyme production. For commercial applications, there have been attempts to develop to produce xylanase in high quantities from inexpensive substrate. Thermomyces lanuginosus is known to produce high levels of. Cellulose-free xylanase in submerged culture using corncob as carbon source (Sing et al, 2000; Puchartet al., 1999). Enzyme production is related to the type and concentration of carbon and nitrogen sources. Several different strategies were applied in order to enhance the enzyme production by various microorganisms (Gupta et al, 2000;Fenget al., 2003). Medium optimization technique is used to increase the enzyme production. This work is integrated to improve the medium for better xylanase production by using different agricultural wastes as the main carbon sources, by Bacillus pumilus under submerged fermentation
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