Abstract
Objective. To observe the curative effect of VAWI on Xinjiang Uygur patients with silicosis fibrosis. Methods. After we diagnosed the 40 patients with the first phase of silicosis, we randomly divided them into two groups: the basic treatment group (group A, n = 20) and the VAWI group (group B, n = 20). At the same time, we selected the age-matched healthy patients (n = 20). We applied the combined protein chip with SELDI-TOF-MS to carry out the serum analysis. The data were analyzed throughout data preprocessing, difference in PEAK screening, hierarchical cluster analysis, and Principal Component Analysis (PCA). We built decision tree model and predict the difference between the PEAK corresponding proteins. Results. The proteins peaks corresponding to name, predicted protein, and gene name were as follows: M2001_69, amyloid beta a4 protein, APP, and M2017_02, amyloid beta a4 protein, APP. The different expression of proteins in patients with silicosis was found before and after with VAWI treatment. The predicted proteins were as follows: M1982_50, amyloid beta a4 protein, APP; M3164_50, fibrinogen alpha chain frag, FGA; M3379_28, fibrinogen alpha chain frag, FGA; and so on. Conclusion. VAWI presented curative effect on patients with silicosis fibrosis via the alternation of proteins expression in serum.
Highlights
The mechanism of pneumoconiosis is a long-term inhalation of productive dust which leads to the pulmonary fibrosis
We aim to study the effect of Vernonia anthelmintica Willd Injection (VAWI) treatment on patients with silicosis fibrosis and provide a future basis for the rational development of novel therapy
Raw data were collected by Ciphergen Protein Chip Software and correction processing, the peak data were determined, mass-to-charge ratio less than 1000 of the peak is substrate peak, filtered based on the peak conduct subsequent data analysis
Summary
The mechanism of pneumoconiosis is a long-term inhalation of productive dust which leads to the pulmonary fibrosis. It is one of the most serious occupational disease hazards in China. Pneumoconiosis has 13 types including silicosis and coal workers pneumoconiosis [1, 2]. Proteomics technology includes purification technology and protein identification technology. Mass spectrometry technology is the core of the protein identification technology [3]. The surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) is a kind of ultramicro, high flux, fully automatic screening protein technology which can detect a variety of samples including serum, urine, and cell [4]
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