Effect of XIAP gene interference on proliferation and apoptosis of human QBC939 cells

Abstract

Objective To study the inhibitory effects of antisense oligodeoxynucleotide (ASODN) on X-linked inhibitor of apoptosis protein (XIAP) gene expression in QBC939 cell line,and the effects on proliferation and apoptosis of QBC939 cells. Methods ASODN was transfected into cell line QBC939 by LipofectamineTM 2000,and transfection efficiency at the 12th,24th,and 48th h after transfec-tion was observed by fluorescence microscopy. Proliferation inhibition rate, XIAP mRNA expression,cell cycle and apoptosis rate of QBC939 cells treated with 1. 11 μmol/L ASODN were assayed by MTT,RT-PCR and flow cytometry (FCM). Results Transfection efficiency was about 95%. In contrast with the control group,the inhibition rate of cell proliferation was about (27.63 ±1.15)% (24 h) and (42.95 ± 1.07) % (48 h) respectively after treatment with 1.11 μmol/L ASODN. The gene expression level of XI-AP in ASODN-transfected group was decreased by 23% (P<0.05). The apoptosis rate and the percent-age of QBC939 cells in G_0/G_1 were increased by 27. 34% and 9. 94% in ASODN-transfected group as compared with control group (P<0. 05). Conclusion The liposomal transfection of ASODN can down-regulate the expression of XIAP gene, which can inhibit proliferation and induce apoptosis of QBC939 cells. Key words: Carcinoma of bile duct; Gene therapy; Apoptosis