Effect of Xiao-ai-ping(XAP) on Adhesion Molecule-1 Kunming Mice Bearing H22

Abstract

Objective To observe the effect of Xiaoaiping on expression of adhesion molecule-1 in mice.Methods Liver cancer cells(H22) were transplanted subcutaneously into Kunming mice right armpit to establish experimental model.50 mice were randomly divided into 5 groups as well as normal group.XAP with 0.01 ml/(g·d),0.02 ml/(g·d) and 0.04 ml/(g·d) doses(low,medium and high doses) was intraperitoneally injected d1~14.The negative control treated by saline,and positive control group was injected 5-Fu by 5~20 mg/(kg·d) d1~7.ELISA was used to test sICAM-1 level,calculation spleen index and inhibition rate.Results The tumor inhibition rates in low,medium and high-dose groups injected with XAP were 20.7%,31.6%,38.63% respectively,and higher than that in negative control group,while the high-dose group was similar to positive control group(45.97%).Compared with negative control group,spleen index in each research groups were increased statistically(P0.05);and similar to positive control group(P0.05).Secretion capacities in Low,medium and high-dose group were(53.26±9.36) ng/L,(35.75±11.16) ng/L,(29.13±8.52)ng/L respectively and more decreased,compared with negative group(P0.05).No obvious difference was observed between the high-dosage group and the positive group(P0.05).Conclusion Xiaoaiping inhibited tumor growth,and improve immune function,through reducing the expression of serum cell adhesion molecule-1 in tumor-bearing mice.