Effect of wounding on ethylene biosynthesis and senescence of detached spinach leaves

Abstract

Parameters of senescence and ethylene biosynthesis pathway were screened simultaneously in detached spinach leaves and leaf discs. Senescence was enhanced by application of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) and was retarded by amino‐ethoxyvinylglycine (AVG). Evidence is presented showing that the bursts of both wound‐ and climacteric‐like ethylene promoted senescence of detached leaves and leaf discs. This ethylene‐enhanced leaf senescence was dependent on: (a) ethylene production rates in the tissue; (b) the degree of wounding. Wounding resulted in elevated levels of 1‐(malonylamino)cyclopropane‐1‐carboxylic acid (MACC), which declined in advanced stages of senescence. The results suggest that wounding might be regarded as one of the primary events in the induction of the senescence syndrome in detached leaves and leaf discs, while ethylene is implicated as a regulator of the rate of the process.