Effect of white tea extract incorporated calcium silicate cements on flexural strength and collagen degradation of dentin

Abstract

ABSTRACT Aim: The aim of the study was to comparatively evaluate the effect of white tea extract-incorporated mineral trioxide aggregate (MTA) and Biodentine (BD) on collagen degradation and flexural strength of coronal dentin. Methods: Two hundred and fifty dentin beams (10 mm × 2 mm × 2 mm) were obtained from the mid-coronal portion of caries-free human 3rd molars. They were randomly assigned to five experimental groups (n = 50). Group 1-control group (No experimental materials placed); Group 2-MTA; Group 3-MTA with 10% white tea extract (WMTA); Group 4-BD; and Group 5-BD with 10% white tea extract (WBD). All experimental materials were mixed and then spread onto the prepared dentin beams. Each group was further divided into five subgroups with each 10 samples according to aging periods of 24 h, 1, 3, 6 months, and 1 year as A, B, C, D, and E, respectively. The dentin beams were placed in contact with a set calcium silicate cement block in silicone molds, with only one side of each beam exposed to the cement at 37°C in a 100% relative humidity chamber. At each designated time period, the beams were copiously rinsed with deionized water and tested for flexural strength and collagen degradation using a universal testing machine and Fourier Transform-Raman spectrometer, respectively. Flexural strength results were statistically analyzed using one-way ANOVA, followed by post hoc adjustment with Tukey’s test for pair-wise comparisons with P < 0.05. Results: Collagen degradation was more in Group 2, followed by 4, 3, and 5. All the groups showed a gradual reduction in flexural strength over 1 year. Group 2 showed the highest reduction in flexural strength over 1 year. Group 5 showed the least reduction in flexural strength. Conclusion: BD and white tea-incorporated BD showed less collagen degradation and better flexural strength than MTA and white tea-incorporated MTA, respectively.