Effect of Wenyang-Yixin recipe on NF-κB signal transduction pathway of myocardial ischemia-reperfusion injury in rats

Abstract

Objective To investigate the influence of Wenyang-Yixin recipe(WYYXR) on NF-κB signal transduction pathway of myocardial ischemia-reperfusion injury in rats. Methods 32 SD rats were divided into 4 groups randomly, sham-operated group, model group, western medicine control group and WYYXR group. Sham-operated group and model group were given physiological saline solution (10 ml/kg),estern medicine control group were treated with fluvastatin (4 mg/kg), and self-made Chinese herb was perfusedinto rats in WYYXR group (1 ml/100 g) each time, once a day, for successive three weeks before model making.The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats. NIK, IKKβ and IκBα protein expression of myocardial tissues in rats were detected by western blotting method. And the levels of NF-κBp65mRNA were determined with RT-PCR. Results Compared with model group, the expressions of NIK, IKKβ and NF-κBp65mRNA were lower, and the protein expression of IκBα was increased in WYYXR group, there were significant differences between the two groups (P=0.004> 0.003, 0.018, 0.004,P<0.05) . Conclusion The Wenyang Yixin Recipe can protect myocardium from MI/RI. Key words: Wenyang-Yixin recipe; Myocardial ischemia-reperfusion injury; Signal transduction pathway