Effect of water activity on the inactivation kinetics of Escherichia coli O157:H7 by electron beam in ground beef, chicken breast meat, and trout fillets

Abstract

SummaryWater activity (aw) of ground beef, chicken breast meat, and trout fillets was modified to intermediate (aw 0.98–0.99) and lowest (aw 0.94–0.96) levels. The meat samples with modified and unaltered (native, aw 1.00) aw were inoculated with Escherichia coli O157:H7 and subjected to electron beam (e‐beam). Survivor curves were plotted and the D10‐values were calculated. The D10‐values ranged from 0.22 kGy for trout at native aw to 0.33 kGy for beef at intermediate, and chicken and trout at lowest aw. Regardless of the species, aw reduction increased E. coli resistance to e‐beam, suggesting that even small depletion of unbound water from food increases survival. The difference of the D10‐values between the samples at intermediate and lowest aw was insignificant. E‐beam could be used before aw‐reducing techniques are applied to food products. However, this would require stringent microbial control following e‐beam processing. The ‘tailing’ of survivors was observed for some samples with reduced aw.