Effect of washing on the post-thaw quality of cryopreserved ram epididymal spermatozoa

Touqeer Ahmed,Farooz Ahmad Lone,Asloob Ahmad Malik,Rafiqul Islam

doi:10.14202/vetworld.2016.519-523

Touqeer Ahmed, Farooz Ahmad Lone + Show 2 more

Open Access

https://doi.org/10.14202/vetworld.2016.519-523

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Abstract

Aim:The aim of the study was to evaluate the effect of washing on the post-thaw quality of ram cauda epididymal spermatozoa (P1: Unwashed, P2: Washed).Materials and Methods:Fresh testicles of adult healthy slaughtered rams were collected and transported to the laboratory in an ice chest, where they were weighed, and cauda epididymides were separated. These cauda epididymides were used for recovery of spermatozoa in tris-citric acid fructose buffer by incision method. Spermatozoa samples showing ≥70% progressive motility were pooled and processed further. The mean values (±standard error) of various parameters such as the percentage of sperm motility, live sperm, intact acrosome, and hypo-osmotic swelling test (HOST) reacted spermatozoa were recorded.Results:In this experiment, the percent sperm motility, live spermatozoa, and intact acrosome both at pre-freeze and post-thaw were higher (p>0.05) in P1 than P2. However, the post-thaw percent HOST reacted spermatozoa was slightly higher (p>0.05) for P2 than P1.Conclusion:Washing of cauda epididymal spermatozoa has no significant adverse effect on the quality during cryopreservation. Therefore, this processing method can be applied wherever necessary before the extension of the recovered spermatozoa sample in different ram extenders.

Highlights

The use of cryopreserved sperm in assisted reproductive techniques has become an indispensable tool for breeding management in domestic animals [1]
Washing of cauda epididymal spermatozoa has no significant adverse effect on the quality during cryopreservation. This processing method can be applied wherever necessary before the extension of the recovered spermatozoa sample in different ram extenders
Each pool was divided into two aliquots, out of which, one aliquot was extended without washing (P1), and another was washed using Tris-citric acid fructose (TCF) buffer (P2)

Summary

Introduction

The use of cryopreserved sperm in assisted reproductive techniques has become an indispensable tool for breeding management in domestic animals [1]. Being an important technique semen cryopreservation is used for the application of assisted reproduction such as artificial insemination and in vitro fertilization, which contribute to increased production and genetic selection [2]. Several researchers have studied the effect of different washing solutions and centrifugations regimes in different species to find out a proper method for improving the quality of cryopreserved semen. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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