Effect of warm ischemia time to the islet function on the non-heart-beating donor rat

Abstract

Objective To observe the influence of warm ischemia time on acquisition of rat pancreatic islets and islet function. Method Male Wistar rats were used. After heart beats stopped, the pancreases in four groups of rats were harvested, and warm ischemia time was 0, 15, 30 and 45 min separately. The pancrease was preserved in UW at 4℃ for 8 h, and subjected to injection of collagenase solutions. After islets were acquired, the purity, survival rate and islet activity were tested, and statistical analysis was performed. Result The number of islets obtained in 0 min group, 15 min group, 30 min group and 45 min group was (433±41), (396±38), (350±31) and (66±17) IEQ/one, islet viability was 94%, 88%, 77% and 25%, and purity was 88%, 78%, 60% and 32%, and insulin release index was 2.38±0.23, 2.25±0.18, 2.19±0.18 and 1.25±0.12, respectively. There was no significant difference in islet number, purity, survival rate and activity 15 min group and 30 min group between 15 min group or 30 min group and 0 min group (P>0.05). There was significant difference between 45 min group and 0 min group in islet number, purity, survival rate and activity (P 75%, and purity >50%). Conclusion Warm ischemia time of 15 min in non-heart-beating brain death(NHBD) rats had no effect on islet isolation and purification. Warm ischemia time within 30 min showed no significant influence on islets of NHBD rats, which can be used in islet transplantation. Warm ischemia time at 45 min showedsignificant influence on islets of NHBD rats, which can’t be used in islet transplantation. Key words: Rat; Islet; Warm ischemia time