Effect of vitamin e on the quality of frozen buck semen

Abstract

A total of 40 ejaculates collected from five Beetal bucks twice weekly were used to study the effect of 0 (control), 2, 5 and 7 mM vitamin E on the quality of frozen semen by split sample technique. The semen extended using Tris was filled in French mini straws (0.25 ml) and sealed with polyvinyl alcohol powder of different colours for identification of semen of different bucks processed with different concentrations of vitamin E. The mean per cent, sperm motility, live sperm, live intact acrosome and hypo-osmotic sperm swelling test (HOST)-reacted sperm were significantly (P<0.05) higher in the extender with 2 mM vitamin E (63.75±0.56, 64.29±0.38, 45.24±0.42 and 52.89±0.23, respectively) than that with 5, 7 mM vitamin E and the control. The release of alanine transaminase and aspartate transaminase from post-thaw spermatozoa was also the lowest (35.67±0.31 and 215.08±0.41 U/L, respectively) in the extender containing 2 mM vitamin E.