Abstract
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of reproductiveaged women. Vitamin E is used in combination with clomid, metformin, melatonin or other drugs to ameliorate and improve the symptoms of PCOS. The aim is to investigate the histological effect of vitamin E on PCOS. PCOS model using dehydroepiandrosterone (DHEA) was adopted. Female mice were divided into eight groups (n = 6). Group 1 was administered with 1% T80; Group 2 was administered with DHEA; Group 3 was administered with clomid; Group 4 was administered with vitamin E; Group 5 was administered with DHEA and vitamin E; drugs were administered for 20 days. Group 6 was administered with DHEA per day for 20 days followed by clomid, a dose per day, for the next 10 days; Group 7 was administered with DHEA per day for 20 days followed by vitamin E, a dose per day, for the next 10 days; Group 8 was administered with DHEA every day for 20 days followed by no treatment for the next 10 days. Mice were sacrificed, at the end of experiment, by neck dislocation, ovary was surgically separated and kept in 10% formalin for histological analysis. DHEA administration produces PCOS changes in ovary. Clomid did not improve PCOS induced by DHEA, while vitamin E ameliorates PCOS to nearly normal. Vitamin E showed marked recovery of the ovarian tissue with the presence of many follicles in the various stages of development, indicating normal oogenesis. Follicles showed normal granulosa layer with defined thecal layers. The presence of corpora lutea was also seen, indicating that vitamin E treatment restore normal estrous cycle.
Highlights
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of reproductiveaged women (Diamanti-Kandarakis et al 1999; Goodman et al 2015)
Puberty is initiated with the maturation of the hypothalamic-pituitary ovarian axis and secretion of gonadotrophin-releasing hormone (GnRH), the activity of which is suppressed during childhood (Roe and Dokras 2011)
The aim of this work is to investigate the histological effect of vitamin E alone on PCOS induced by dehydroepiandrosterone (DHEA) using female albino mice
Summary
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder of reproductiveaged women (Diamanti-Kandarakis et al 1999; Goodman et al 2015). This syndrome includes various criteria such as hyperandrogenism (Goodman et al 2015; Lerchbaum et al 2014), menstrual irregularity, oligomenorrhea, amenorrhea, infertility (Diamanti-Kandarakis et al 1999; Pannill 2002), and polycystic ovary morphology (Goodman et al 2015; Lerchbaum et al 2014). PCOS is associated with obesity and metabolic abnormalities, such as dyslipidemia and insulin resistance. It can be clinically expressed with acne, hirsutism or androgen-dependent alopecia (Diamanti-Kandarakis et al 1999) and elevated serum luteinising hormon (LH) levels (The Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group 2004). The pathophysiology of the PCOS encompasses inherent ovarian dysfunction that is strongly influenced by external factors, such as disturbances of the hypothalamic-pituitary-ovarian axis and hyperinsulinemia, these disturbances affects both ovarian androgen production and oocyte development (Balen 2004)
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