Abstract
The purpose of this research was to evaluate the post-thawing quality (sperm motility, mortality and abnormality) of frozen-thawed Simmental bull semen with the addition of vitamin E in the extender. The material used for research were semen from two selected Simmental bulls. Vitamin E as treatment in addition to the extender consisted of T0 (no addition of vitamin E), T1 (0,134 gram/100 ml extender), T2 (0,268 gram/100 ml extender) and T3 (0,402 gram/100 ml extender). Post-thawing evaluation conducted 24 hours after the freezing process and observed after the thawing process in 37°C water bath for 30 seconds. Parameters observed in this research were the post-thawing quality of frozen-thawed Simmental bull semen based on motility, mortality and abnormality percentage. Sperm motility evaluated using a microscope with 100x and 400x magnifying, sperm mortality observed using 400x magnifying and counted based on 0,2% eosin-negrosin staining, sperm abnormality observed using 400x magnifying and counted based on the number of morphologically abnormal sperm cells. Semen post-thawing motility was not significantly affected by vitamin E addition of T1, T2 and T3 (P<0,05). T1 and T2 were able to significantly lower the mortality percentage compared to T0 and T3 (P<0,05). T1 and T2 were also very significantly affecting the decreased of abnormality percentage compared to T0 and T3 (P<0,01). T1 generally gave the best result on the improvement of post-thawing motility and decreased the percentage of sperm mortality and abnormality.
Highlights
Artificial insemination center has been established on several provinces in Indonesia in order to fulfill the necessity of frozen semen for artificial insemination process
Several factors that could determine the quality of frozen semen are the genetic quality of bull, semen production process and implementation of artificial insemination [1]
Zubair et al reported that vitamin E as an antioxidant had proven capable of mitigating the effect of reaction with reactive oxygen species (ROS) dan lipid peroxidation (LPO) spermatozoa membrane so that semen quality will be preserved during the whole production process or cold storage [3, 12]
Summary
Artificial insemination center has been established on several provinces in Indonesia in order to fulfill the necessity of frozen semen for artificial insemination process. Lipid peroxidation on spermatozoa will result in the loss of motility, decrease in cell fructolysis and respiration activity and leakage of cell fluid through damaged membrane Antioxidants such as vitamin E are known for their ability to give protection to the sperm membrane by inhibiting the formation of ROS and lipid peroxidation [2]. The main purpose of this research was to evaluate the post-thawing quality (sperm motility, mortality, and abnormality) of frozen-thawed Simmental bull semen with the addition of vitamin E in the extender. This research might have the benefit of increasing the postthawing quality of Simmental bull semen after the addition of vitamin E in semen extender and resulting in the increase of frozen semen production at the artificial insemination center
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