Abstract
The purpose of the experiments reported here was to ascertain whether or not vitamin A is one of the substances needed by fibroblasts for their life and proliferation in vitro. Two previous investigators have already attempted to answer this question. Unfortunately, however, they arrived at opposite conclusions. Thus, Burrows1 compared the change in concentration of vitamin A in chick embryos, as they increased in age, with the change occurring simultaneously in the growth-promoting power of the juice of the embryos. He concluded that vitamin A inhibited growth. Bisceglie,2 on the other hand, added a trace of the “A factor” to hanging drop cultures of liver and spleen and found it to be growth stimulating. No information was given as to the source of the “A factor”, or the extent to which it had been purified.In the present investigation, crystalline carotene was first tried as a source of vitamin for the cells. No increase in growth was obtained.∗ Vitamin A was, therefore, prepared from halibut liver oil...
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