Abstract
Incubation of human serum solutions low in glyceride content for 16 hr resulted in a net increase of cholesterol esters in all three major human serum lipoprotein classes, namely the very low-density lipoproteins of d When serum was incubated in the presence of high concentrations of VLDL lipoproteins, the increase in cholesterol esters was found only in the VLDL fraction. The VLDL apparently took up newly-formed cholesterol esters produced by serum fatty acid transferase activity, and accepted some cholesterol esters initially bound to the LDL and HDL. When the transferase was inhibited with sodium p-hydroxymercuribenzoate, some of the cholesterol esters initially bound to the LDL and HDL were still transferred to the VLDL. The HDL class was then the principal contributor of the cholesterol esters. These observations indicate that reciprocal transfer of cholesterol esters for glycerides in human serum lipoproteins can occur.
Highlights
SUMMAR,Y Incubation of human serum solutions low in glyceride content for 16 hr resulted in a net increase of cholesterol esters in all three major human serum lipoprotein classes, namely the very low-densitylipoproteinsof d < 1.006 (VLDL), low-density lipoproteins of d 1.006-1.063 (LDL) and highdensity lipoproteins of d 1.063-1.21 (HDL)
When the transferase was inhibited with sodium p-hydroxymercuribenzoate, some of the cholesterol esters initially bound to the LDL and HDL were still transferred to the VLDL
I n the present paper, we report our studies, in detail, on lipid transfer between all major human serum lipoprotein classes, the VLDL, LDL, and HDL in sera incubated with large amounts of added human VLDL lipoproteins
Summary
SUMMAR,Y Incubation of human serum solutions low in glyceride content for 16 hr resulted in a net increase of cholesterol esters in all three major human serum lipoprotein classes, namely the very low-densitylipoproteinsof d < 1.006 (VLDL), low-density lipoproteins of d 1.006-1.063 (LDL) and highdensity lipoproteins of d 1.063-1.21 (HDL). The HDL class was the principal contributor of the cholesterol esters These observations indicate that reciprocal transfer of cholesterol esters for glycerides in human serum lipoproteins can occur. I n the present paper, we report our studies, in detail, on lipid transfer between all major human serum lipoprotein classes, the VLDL, LDL (low-density lipoproteins of d 1.006-1.063), and HDL in sera incubated with large amounts of added human VLDL lipoproteins. I n addition, preliminary data are presented on the influence of inhibition of the cholesterol esterifying enzyme of human serum-the serum fatty acid transferase as described by Glomset (4)-on this lipid transfer
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