Effect of various solvent fractions on antioxidant potential of glycyrrhiza glabraL. (Mulhatti) Roots

Abstract

The effect of widely used methods and solvents on the antioxidant activity of Mulhatti root has been studied. The dry powder of Mulhatti root is extracted in methanol and by liquid-liquid separation of different solvent fractions, namely dichloromethane (DCM), ethyl acetate (EA), n-butanol (nB), residual aqueous solution (RA) and non-aqueous (NAR) residues were prepared. The antioxidant activity of all the different solvent fractions of Mulhatti root was evaluated using antioxidant assays such as the 2,2 diphenyl-1-picryl hydrazyl free radical scavenger assay and the total antioxidant activity. Total phenol and flavonoid content was also measured. For methanol extraction, the aqueous fraction was considered to be the best method for phenol and flavonoid extraction, but the ethyl acetate fraction showed the lowest IC50 (41.08), followed by DCM (41.77), non-aqueous residues (64.50), n-butanol (351.78) and 461.50 μg/mL in residual water. The outcomes confirmed that the extracting solvent considerably altered the antioxidant of Mulhatti roots.