Abstract
Siderophores are low molecular weight compounds secreted by bacteria that 
 bind ferric iron with extremely high affinity. The siderophores help bacteria to 
 meet their iron needs under stress conditions as these compounds help in the 
 transport and storage of soluble form of iron in cells. In current study, we report 
 the effect of various physicochemical parameters that influence siderophore 
 production in marine Pseudomonas aeruginosa MGPB31. Siderophore 
 production was assayed using Chrome Azurol S (CAS) shuttle assay. The 
 maximum siderophore production (56% SU) was obtained in minimal M9 
 medium after 72 h incubation, followed by succinate medium (52% SU) and 
 King’s medium (51% SU). The M9 medium was then modified by replacing its 
 carbon, nitrogen and phosphate source. Enhanced siderophore production was 
 observed in modified medium comprising of glycerol (70% SU), tri ammonium 
 citrate (67% SU) and KH2PO4 (64% SU). The medium supplemented with iron 
 exhibited a significant reduction in siderophore production. The effect of 
 physical parameters like temperature, pH and agitation on siderophore 
 production revealed that the isolate exhibited maximum siderophore production 
 under static conditions in modified M9 medium devoid of iron at 25 ± 2°C and 
 neutral pH. The enhanced siderophore production obtained by marine P. 
 aeruginosa MGPB31 indicates its further applicability in fields of industry 
 agriculture and environment.
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