Abstract
In vitro culture of plant species most commonly uses fluorescent lamps as a light source. Other sources of illumination, LEDs (Light-emitting diodes) have proven to be more efficient for in vitro culture. In the conducted experiment, the effect of various LEDs on the in vitro morphogenesis, proliferation of shoots, growth & rooting of Banana (Musa acuminata) cv. Grande Naine was observed. For this purpose, 5 different sources of light were tested under a 16-h photoperiod: fluorescent lamps (Fl), white (W), red (R), blue (B), and B/R (1:1). The proliferation rate was higher with R LEDs compared with Fl light, although shoots have a lower length under R LEDs. Under B/R LEDs, maximum shoot elongation was obtained. LEDs did not enhance the rooting of shoots but increased the photosynthetic pigments content under B/R, which contributed to the acclimation process of in vitro plantlets. Our results revealed that the spectrum of different light sources produced different effects during the in vitro cloning of Grande naine.
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