Effect of various ionic compositions upon the membrane potentials during activation of sea urchin eggs

Abstract

The membrane potentials of sea urchin ( Hemicentrotus pulcherrimus) eggs before and after fertilization and their changes during the membrane elevation induced by intracellular electrical stimulation were recorded in solutions of various ionic compositions. Upon fertilization, the membrane potential (−10 mV) depolarized and reversed polarity by a few mV, then gradually returned to a new steady level ranging between −50 and −60 mV. The activation potential is closely associated with a transient increase in the membrane permeability. The potential of the unfertilized egg is hyperpolarized by monovalent anions (Br −, Cl − and NO 3 −) and depolarized slightly by K +. In contrast, the membrane of the fertilized egg is markedly depolarized by K +. Suppression of depolarization associated with an increase of the membrane permeability was recorded in Na-free medium (Tris-HCl). The selective increase in permeability to monovalent anions is thought to alternate with the selective increase in permeability to K +through the mediation of a transient increase of Na +-permeability at the time of fertilization. No causal relationship between the membrane elevation and the depolarization was established because the breakdown of the cortical granules occurs without depolarization or an increase in membrane permeability.